Journal article Open Access

Proteome consistency of the aleurone layer in grain of major wheat species grown over different years

Meziani, Samira; Nadaud, Isabelle

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  <identifier identifierType="DOI">10.5281/zenodo.3974052</identifier>
      <creatorName>Meziani, Samira</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="">0000-0002-4027-9670</nameIdentifier>
      <affiliation>Laboratory of Biotoxicology, Djillali Liabes University, Faculty of Life and Natural Sciences, Department of Biology, Algeria</affiliation>
      <creatorName>Nadaud, Isabelle</creatorName>
      <affiliation>UMR. INRA GDEC-UCA 1095, 5 Chemin de Beaulieu, 63000 Clermont-Ferrand, France</affiliation>
    <title>Proteome consistency of the aleurone layer in grain of major wheat species grown over different years</title>
    <subject>aleurone layer</subject>
    <subject>metabolic pathways</subject>
    <date dateType="Issued">2020-08-11</date>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
    <alternateIdentifier alternateIdentifierType="url"></alternateIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3974051</relatedIdentifier>
    <rights rightsURI="">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
    <description descriptionType="Abstract">&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Aleurone layer (AL), being a living cell layer among the peripheral layers of the grain structure obtained after milling wheat, is rich in, vitamins, minerals, and antioxidants potentially nutritional value of the flour. &lt;strong&gt;Objectives: &lt;/strong&gt;To isolate AL in the mature grain of the three major species; Common wheat (CW)&lt;em&gt;, &lt;/em&gt;Durum wheat (DW), and Einkorn wheat (EW) that were grown at two different years as well as to analyze and compare their proteomes revealed through two-dimensional electrophoresis (2DE) and image analysis. &lt;strong&gt;Methods&lt;/strong&gt;: The AL was hand dissected and unicellular purity verified using scanning electron microscopy. AL proteins were separated using IEF pH3-10 X SDS-PAGE then Coomassie-stained. The gels were scanned and the images were compared using Samespot (Nonlinear Dynamics) and were proteins identified using mass spectrometry and database interrogation. &lt;strong&gt;Results&lt;/strong&gt;: For CW and DW samples, no significant quantitative or qualitative differences were observed between the AL proteome in the two years. However, a few quantitative differences were revealed between EW for AL of 2006 and 2007. The &amp;nbsp;identified proteins were classified in the carbohydrate pathway and stress defense response. &lt;strong&gt;Conclusion:&lt;/strong&gt; This remarkable stability over environmental growing conditions strengthens the need to pay greater attention to this unicellular living cell of the wheat grain.&lt;/p&gt;</description>
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