Journal article Open Access

Proteome consistency of the aleurone layer in grain of major wheat species grown over different years

Meziani, Samira; Nadaud, Isabelle

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  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.3974052", 
  "container_title": "The North African Journal of Food and Nutrition Research", 
  "language": "eng", 
  "title": "Proteome consistency of the aleurone layer in grain of major wheat species grown over different years", 
  "issued": {
    "date-parts": [
  "abstract": "<p><strong>Background: </strong>Aleurone layer (AL), being a living cell layer among the peripheral layers of the grain structure obtained after milling wheat, is rich in, vitamins, minerals, and antioxidants potentially nutritional value of the flour. <strong>Objectives: </strong>To isolate AL in the mature grain of the three major species; Common wheat (CW)<em>, </em>Durum wheat (DW), and Einkorn wheat (EW) that were grown at two different years as well as to analyze and compare their proteomes revealed through two-dimensional electrophoresis (2DE) and image analysis. <strong>Methods</strong>: The AL was hand dissected and unicellular purity verified using scanning electron microscopy. AL proteins were separated using IEF pH3-10 X SDS-PAGE then Coomassie-stained. The gels were scanned and the images were compared using Samespot (Nonlinear Dynamics) and were proteins identified using mass spectrometry and database interrogation. <strong>Results</strong>: For CW and DW samples, no significant quantitative or qualitative differences were observed between the AL proteome in the two years. However, a few quantitative differences were revealed between EW for AL of 2006 and 2007. The &nbsp;identified proteins were classified in the carbohydrate pathway and stress defense response. <strong>Conclusion:</strong> This remarkable stability over environmental growing conditions strengthens the need to pay greater attention to this unicellular living cell of the wheat grain.</p>", 
  "author": [
      "family": "Meziani, Samira"
      "family": "Nadaud, Isabelle"
  "page": "280-284", 
  "volume": "04", 
  "type": "article-journal", 
  "issue": "07", 
  "id": "3974052"
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