September 1, 2020 Journal article Open Access

Uncu, Andrei

MARC21 XML Export

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    <subfield code="a">high-pressure liquid chromatography</subfield>
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    <subfield code="a">propylthiohinothiadiazole</subfield>
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    <subfield code="u">Nicolae Testemitsanu State University of Medicine and Pharmacy, Chisinau, the Republic of Moldova</subfield>
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    <subfield code="a">Development and validation of the high-pressure liquid chromatographic method for the quantitative determination of propylthiohinothiadiazole</subfield>
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    <subfield code="a">&lt;p&gt;&lt;strong&gt;Background:&lt;/strong&gt; Propylthiohinothiadiazole is a thiadiazole derivative with pronounced antimycobacterial action. Substance is researched in order to develop methods of analysis and standardization, analytical documents regulating quality for use as an active principle in the development of pharmaceutical forms.&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Material and methods:&lt;/strong&gt; 3 series of propylthiohinothiadiazole, internal reference standard of 2-(propylthio)-5H-[1,3,4]-thiadiazole[2,3-b]-quinazolin5-one substance with concentration 99.98%, chromatographic system Shimadzu LC-20AD high-pressure liquid chromatography (HPLC) with UV-VIS detector, acetonitrile and dimethylsulfoxide of purity grade &amp;ldquo;pro HPLC analysis&amp;rdquo; (Sigma Aldrih and Merck), bidistilled purified water.&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Results:&lt;/strong&gt; Linearity is proved for concentrations of 5-30 &amp;micro;g/ml, the linear regression equation is y=36472x-44580, R&amp;sup2;=0.9999. The limit of detection is 0.729 &amp;micro;g/ml and the limit of quantification is 2.210 &amp;micro;g/ml. It was established that the method is accurate (mean recovery values at 80%, 100% and 120% concentration levels were close to 100%). The accuracy of the method was expressed by repeatability and intermediate accuracy. The variation of the chromatographic conditions established that the method is robust. For all validation parameters, relative standard deviation was less than 1.&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; The validation results show that the developed HPLC method is simple, fast, accurate and reproducible.&lt;/p&gt;</subfield>
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