3906054
doi
10.1021/acsnano.9b08824
oai:zenodo.org:3906054
user-eu
José Lifante
Nanobiology Group, Instituto Ramón y Cajal de Investigación Sanitaria, IRYCIS, Ctra. Colmenar km. 9.100, Madrid 28034, Spain
Nuria Fernandez
Fluorescence Imaging Group, Departamento de Fisiologı́a, Facultad de Medicina, Universidad Autónoma de Madrid, Avda. Arzobispo Morcillo 2, Madrid 28029, Spain
Daniel Jaque
Fluorescence Imaging Group, Departamento de Fisiologı́a, Facultad de Medicina, Universidad Autónoma de Madrid, Avda. Arzobispo Morcillo 2, Madrid 28029, Spain
Erving Ximendes
Nanobiology Group, Instituto Ramón y Cajal de Investigación Sanitaria, IRYCIS, Ctra. Colmenar km. 9.100, Madrid 28034, Spain
In Vivo Spectral Distortions of Infrared Luminescent Nanothermometers Compromise Their Reliability
Yingli Shen
Fluorescence Imaging Group, Departamento de Fı́sica de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, Madrid 28049, Spain
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
luminescence thermometry
attenuation
scattering
thermal reading
absorption
<p>Luminescence nanothermometry has emerged over the last decade as an exciting field of research due to its potential applications where conventional methods have demonstrated to be ineffective. Preclinical research has been one of the areas that have benefited the most from the innovations proposed in the field. Luminescent nanothermometers have starred unimaginable advances such as in vivo intratumoral thermal reading or brain activity monitoring through real-time thermal sensing. Nevertheless, certain questions concerning the reliability of the technique under in vivo conditions have been continuously neglected by most of the scientific community. In this work, hyperspectral in vivo imaging demonstrates that in vivo luminescent nanothermometry is not as reliable as previously thought. This work, indeed, reveals how the temperature-dependent optical transmittance of living tissues can induce spectral changes in the measured fluorescence. These changes, in turns, can be wrongly attributed to temperature variations. The next steps that should be taken in the future for a reliable in vivo luminescence nanothermometry are discussed together with a perspective view of the field after the findings that are here reported. </p>
Zenodo
2020-03-31
info:eu-repo/semantics/preprint
3906053
user-eu
award_title=Nanoparticles-based 2D thermal bioimaging technologies; award_number=801305; award_identifiers_scheme=url; award_identifiers_identifier=https://cordis.europa.eu/projects/801305; funder_id=00k4n6c32; funder_name=European Commission;
1593037102.77669
1695816
md5:050107fb9fab27434ea4b4d0b8773d38
https://zenodo.org/records/3906054/files/Shen_et_al_ACSNano_Zenodo.pdf
1514
md5:fe3307edb13a0554be83954a72a3eaf5
https://zenodo.org/records/3906054/files/Fig6b.dat
1544
md5:fe669c41783c4ef93a9641975554c24b
https://zenodo.org/records/3906054/files/Fig6a.dat
1907
md5:4b54d506702a7bdf1d29e7406d614cab
https://zenodo.org/records/3906054/files/Fig6c.dat
614
md5:dc6a082bb1d2c44628d0c25622233d58
https://zenodo.org/records/3906054/files/Fig5d.dat
public