June 4, 2020 Technical note Open Access

Nicouleau, Michael; Pimentel, Luisa; Shlaifer, IrIna; Durcan, Thomas M.

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    <subfield code="a">&lt;p&gt;This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:&lt;/p&gt;

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	&lt;li&gt;CRISPR-Cas9 editing;&lt;/li&gt;
	&lt;li&gt;Droplet digital PCR (ddPCR) technology;&lt;/li&gt;
	&lt;li&gt;Single cell sorting.&lt;/li&gt;
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