Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology

Nicouleau, Michael; Pimentel, Luisa; Shlaifer, IrIna; Durcan, Thomas M.


MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="041" ind1=" " ind2=" ">
    <subfield code="a">eng</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">CRISPR</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">ddPCR</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">knockout</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">gene editing</subfield>
  </datafield>
  <controlfield tag="005">20200619211142.0</controlfield>
  <controlfield tag="001">3875777</controlfield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">MNI, McGill University</subfield>
    <subfield code="a">Pimentel, Luisa</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">MNI, McGill University</subfield>
    <subfield code="a">Shlaifer, IrIna</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Durcan, Thomas M.</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">2349443</subfield>
    <subfield code="z">md5:3657930ba2b4db733db4a31c7f1a62de</subfield>
    <subfield code="u">https://zenodo.org/record/3875777/files/EDDU-013-01_CRISPR editing of non-iPSCs through ddPCR_v1.0.pdf</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2020-06-04</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire</subfield>
    <subfield code="p">user-sgc-opennotebook</subfield>
    <subfield code="o">oai:zenodo.org:3875777</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">MNI, McGill University</subfield>
    <subfield code="a">Nicouleau, Michael</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-sgc-opennotebook</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">https://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:&lt;/p&gt;

&lt;ul&gt;
	&lt;li&gt;CRISPR-Cas9 editing;&lt;/li&gt;
	&lt;li&gt;Droplet digital PCR (ddPCR) technology;&lt;/li&gt;
	&lt;li&gt;Single cell sorting.&lt;/li&gt;
&lt;/ul&gt;</subfield>
  </datafield>
  <datafield tag="773" ind1=" " ind2=" ">
    <subfield code="n">doi</subfield>
    <subfield code="i">isVersionOf</subfield>
    <subfield code="a">10.5281/zenodo.3875776</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.5281/zenodo.3875777</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">publication</subfield>
    <subfield code="b">technicalnote</subfield>
  </datafield>
</record>
308
232
views
downloads
All versions This version
Views 308308
Downloads 232232
Data volume 545.1 MB545.1 MB
Unique views 283283
Unique downloads 215215

Share

Cite as