June 4, 2020 Technical note Open Access

Nicouleau, Michael; Pimentel, Luisa; Shlaifer, IrIna; Durcan, Thomas M.

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  <dc:creator>Nicouleau, Michael</dc:creator>
  <dc:creator>Pimentel, Luisa</dc:creator>
  <dc:creator>Shlaifer, IrIna</dc:creator>
  <dc:creator>Durcan, Thomas M.</dc:creator>
  <dc:date>2020-06-04</dc:date>
  <dc:description>This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:


	CRISPR-Cas9 editing;
	Droplet digital PCR (ddPCR) technology;
	Single cell sorting.
</dc:description>
  <dc:identifier>https://zenodo.org/record/3875777</dc:identifier>
  <dc:identifier>10.5281/zenodo.3875777</dc:identifier>
  <dc:identifier>oai:zenodo.org:3875777</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>doi:10.5281/zenodo.3875776</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/sgc-opennotebook</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>https://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>CRISPR</dc:subject>
  <dc:subject>ddPCR</dc:subject>
  <dc:subject>knockout</dc:subject>
  <dc:subject>gene editing</dc:subject>
  <dc:title>Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology</dc:title>
  <dc:type>info:eu-repo/semantics/technicalDocumentation</dc:type>
  <dc:type>publication-technicalnote</dc:type>
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