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Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology

Nicouleau, Michael; Pimentel, Luisa; Shlaifer, IrIna; Durcan, Thomas M.


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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p>This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:</p>\n\n<ul>\n\t<li>CRISPR-Cas9 editing;</li>\n\t<li>Droplet digital PCR (ddPCR) technology;</li>\n\t<li>Single cell sorting.</li>\n</ul>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Nicouleau, Michael"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Pimentel, Luisa"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Shlaifer, IrIna"
    }, 
    {
      "@type": "Person", 
      "name": "Durcan, Thomas M."
    }
  ], 
  "headline": "Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2020-06-04", 
  "url": "https://zenodo.org/record/3875777", 
  "version": "V1.0", 
  "keywords": [
    "CRISPR", 
    "ddPCR", 
    "knockout", 
    "gene editing"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.3875777", 
  "@id": "https://doi.org/10.5281/zenodo.3875777", 
  "@type": "ScholarlyArticle", 
  "name": "Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology"
}
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