Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology

Nicouleau, Michael; Pimentel, Luisa; Shlaifer, IrIna; Durcan, Thomas M.


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    "description": "<p>This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:</p>\n\n<ul>\n\t<li>CRISPR-Cas9 editing;</li>\n\t<li>Droplet digital PCR (ddPCR) technology;</li>\n\t<li>Single cell sorting.</li>\n</ul>", 
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