Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology

Nicouleau, Michael; Pimentel, Luisa; Shlaifer, IrIna; Durcan, Thomas M.


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  <identifier identifierType="DOI">10.5281/zenodo.3875777</identifier>
  <creators>
    <creator>
      <creatorName>Nicouleau, Michael</creatorName>
      <givenName>Michael</givenName>
      <familyName>Nicouleau</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Pimentel, Luisa</creatorName>
      <givenName>Luisa</givenName>
      <familyName>Pimentel</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Shlaifer, IrIna</creatorName>
      <givenName>IrIna</givenName>
      <familyName>Shlaifer</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Durcan, Thomas M.</creatorName>
      <givenName>Thomas M.</givenName>
      <familyName>Durcan</familyName>
    </creator>
  </creators>
  <titles>
    <title>Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2020</publicationYear>
  <subjects>
    <subject>CRISPR</subject>
    <subject>ddPCR</subject>
    <subject>knockout</subject>
    <subject>gene editing</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2020-06-04</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Technical note</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/3875777</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3875776</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/sgc-opennotebook</relatedIdentifier>
  </relatedIdentifiers>
  <version>V1.0</version>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:&lt;/p&gt;

&lt;ul&gt;
	&lt;li&gt;CRISPR-Cas9 editing;&lt;/li&gt;
	&lt;li&gt;Droplet digital PCR (ddPCR) technology;&lt;/li&gt;
	&lt;li&gt;Single cell sorting.&lt;/li&gt;
&lt;/ul&gt;</description>
  </descriptions>
</resource>
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