Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology

Nicouleau, Michael; Pimentel, Luisa; Shlaifer, IrIna; Durcan, Thomas M.


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.3875777", 
  "language": "eng", 
  "title": "Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology", 
  "issued": {
    "date-parts": [
      [
        2020, 
        6, 
        4
      ]
    ]
  }, 
  "abstract": "<p>This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:</p>\n\n<ul>\n\t<li>CRISPR-Cas9 editing;</li>\n\t<li>Droplet digital PCR (ddPCR) technology;</li>\n\t<li>Single cell sorting.</li>\n</ul>", 
  "author": [
    {
      "family": "Nicouleau, Michael"
    }, 
    {
      "family": "Pimentel, Luisa"
    }, 
    {
      "family": "Shlaifer, IrIna"
    }, 
    {
      "family": "Durcan, Thomas M."
    }
  ], 
  "version": "V1.0", 
  "type": "article", 
  "id": "3875777"
}
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