Technical note Open Access

Uptake of α-Synuclein Preformed Fibrils (PFFs) in Dopaminergic Neural Progenitor Cells

Krahn, Andrea; Reintsch, Wolfgang; Cai, Eddie; Durcan, Thomas M.


JSON-LD (schema.org) Export

{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p>The uptake and accumulation of pathological &alpha;-synuclein aggregates in neuronal cells is thought to be a major underlying mechanism in the pathogenesis of Parkinson&rsquo;s disease. This protocol describes an assay to quantify the uptake of these aggregates by neuronal cells by monitoring the uptake of fluorescently labelled &alpha;-synuclein preformed fibrils (PFFs) in dopaminergic (DA) neural progenitor cells (NPCs) derived from induced pluripotent stem cells (iPSCs). This protocol provides the details and methods we used for other researchers to screen small molecule libraries for actives that modulate the uptake of fluorescently labelled PFFs uptake in NPCs. It describes the assay, subsequent image acquisition, and image analysis by high-content screening (HCS).</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Krahn, Andrea"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Reintsch, Wolfgang"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Cai, Eddie"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Durcan, Thomas M."
    }
  ], 
  "headline": "Uptake of \u03b1-Synuclein Preformed Fibrils (PFFs) in Dopaminergic Neural Progenitor Cells", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2020-06-04", 
  "url": "https://zenodo.org/record/3875761", 
  "version": "V1.0", 
  "keywords": [
    "alpha-synuclein", 
    "high content screening", 
    "neuronal progenitors", 
    "dopaminergic neurons", 
    "Preformed fibrils (PFFs)"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.3875761", 
  "@id": "https://doi.org/10.5281/zenodo.3875761", 
  "@type": "ScholarlyArticle", 
  "name": "Uptake of \u03b1-Synuclein Preformed Fibrils (PFFs) in Dopaminergic Neural Progenitor Cells"
}
143
74
views
downloads
All versions This version
Views 143143
Downloads 7474
Data volume 173.3 MB173.3 MB
Unique views 137137
Unique downloads 6969

Share

Cite as