Sardari, Veronica
Pantea, Valeriana
Gulea, Aurelian
Tagadiuc, Olga
Andronache, Lilia
Svet, Inna
Tapcov, Victor
Gudumac, Valentin
2020-05-30
<p><strong>Background: </strong>Thiol-disulfide metabolism is essential for normal function of the organism. Thus the interest of the scientists in this area of research continues to grow.</p>
<p><strong>Material and methods: </strong>Copper coordination compounds (CCC), derivatives of thiosemicarbaside (CMD-4, CMJ-33, CMT-67), action on thiol-disulfide metabolism in the healthy <em>Ratta albicans</em> kidneys were studied. The animals were divided in 6 groups of 7 rats each. The control group included healthy rats which were injected i/m physiological solution 3 times a week, for 30 days. The rats from groups 2-6 have got 3 times a week, for 30 days, i/m injections of CCC. The activity of following thiol-disulfide metabolism enzymes in the renal supernatant has been measured: glutathion-reductase (GR), glutathion-peroxidase (GPO), glutathion-S-transferase (G-S-T), γ-glutamyl transpeptidase (γ-GTP), glutaredoxin (Grx), as well the amount of the protein SH-groups and of the total glutathione, reduced glutathione (GSH) and oxidized glutathione (GSSG) in renal tissue.</p>
<p><strong>Results: </strong>The compounds exhibit different actions: CMT-67 in the dose of 0.1 µM/kg influenced the activity of the glutathione metabolism enzymes – activated γ-glutamyl transpeptidase (γ-GTP) and glutaredoxine (Grx) and inhibited glutathione reductase (GR), while CMD-4 in doses of 0.1 µM/kg and 1.0 µM/kg and CMJ-33 in the dose of 1.0 µM/kg significantly diminished the reduced glutathione (GSH) level and increased the amount of the oxidised one (GSSG).</p>
<p><strong>Conclusions: </strong>Selective action of the copper coordination copounds established by this study opens new possibilities of their usage in the therapy of kidney diseases.</p>
https://doi.org/10.5281/zenodo.3865976
oai:zenodo.org:3865976
eng
Zenodo
https://zenodo.org/communities/mmj
https://doi.org/10.5281/zenodo.3865975
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Moldovan Medical Journal, 63 (2), 12-17, (2020-05-30)
thiol-disulfide metabolism
copper coordination compounds
kidney tissue
Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compounds
info:eu-repo/semantics/article