Technical note Open Access

Sequencing Analysis Guide

Nicouleau, Michael; Durcan, Thomas M.


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    <subfield code="a">&lt;p&gt;Sanger sequencing is a method of DNA sequencing that is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.&lt;/p&gt;

&lt;p&gt;Sanger sequencing analysis software packages are used for base calling (process of assigning&amp;nbsp;&lt;a href="https://en.wikipedia.org/wiki/Nucleobase"&gt;nucleobases&lt;/a&gt;&amp;nbsp;to&amp;nbsp;&lt;a href="https://en.wikipedia.org/wiki/Chromatogram"&gt;chromatogram&lt;/a&gt;&amp;nbsp;peaks), sequence alignment, trace visualization and variant detection.&lt;/p&gt;

&lt;p&gt;In this protocol, we describe our method used for analyzing sequence data obtained with our Applied Biosystems SeqStudio Genetic Analyzer instrument and analyzed using SnapGene software.&lt;/p&gt;</subfield>
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