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Sequencing Analysis Guide

Nicouleau, Michael; Durcan, Thomas M.


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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p>Sanger sequencing is a method of DNA sequencing that is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.</p>\n\n<p>Sanger sequencing analysis software packages are used for base calling (process of assigning&nbsp;<a href=\"https://en.wikipedia.org/wiki/Nucleobase\">nucleobases</a>&nbsp;to&nbsp;<a href=\"https://en.wikipedia.org/wiki/Chromatogram\">chromatogram</a>&nbsp;peaks), sequence alignment, trace visualization and variant detection.</p>\n\n<p>In this protocol, we describe our method used for analyzing sequence data obtained with our Applied Biosystems SeqStudio Genetic Analyzer instrument and analyzed using SnapGene software.</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Nicouleau, Michael"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Durcan, Thomas M."
    }
  ], 
  "headline": "Sequencing Analysis Guide", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2020-05-07", 
  "url": "https://zenodo.org/record/3810825", 
  "version": "V1.0", 
  "keywords": [
    "sequencing", 
    "snapgene", 
    "DNA analysis"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.3810825", 
  "@id": "https://doi.org/10.5281/zenodo.3810825", 
  "@type": "ScholarlyArticle", 
  "name": "Sequencing Analysis Guide"
}
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