May 7, 2020 Technical note Open Access

Nicouleau, Michael; Durcan, Thomas M.

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    "description": "<p>Sanger sequencing is a method of DNA sequencing that is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.</p>\n\n<p>Sanger sequencing analysis software packages are used for base calling (process of assigning&nbsp;<a href=\"https://en.wikipedia.org/wiki/Nucleobase\">nucleobases</a>&nbsp;to&nbsp;<a href=\"https://en.wikipedia.org/wiki/Chromatogram\">chromatogram</a>&nbsp;peaks), sequence alignment, trace visualization and variant detection.</p>\n\n<p>In this protocol, we describe our method used for analyzing sequence data obtained with our Applied Biosystems SeqStudio Genetic Analyzer instrument and analyzed using SnapGene software.</p>", 
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    "title": "Sequencing Analysis Guide", 
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