May 7, 2020 Technical note Open Access

Nicouleau, Michael; Durcan, Thomas M.

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  <identifier identifierType="DOI">10.5281/zenodo.3810825</identifier>
  <creators>
    <creator>
      <creatorName>Nicouleau, Michael</creatorName>
      <givenName>Michael</givenName>
      <familyName>Nicouleau</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Durcan, Thomas M.</creatorName>
      <givenName>Thomas M.</givenName>
      <familyName>Durcan</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Sequencing Analysis Guide</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2020</publicationYear>
  <subjects>
    <subject>sequencing</subject>
    <subject>snapgene</subject>
    <subject>DNA analysis</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2020-05-07</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Technical note</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/3810825</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3810824</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/sgc-opennotebook</relatedIdentifier>
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  <version>V1.0</version>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
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  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Sanger sequencing is a method of DNA sequencing that is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.&lt;/p&gt;

&lt;p&gt;Sanger sequencing analysis software packages are used for base calling (process of assigning&amp;nbsp;&lt;a href="https://en.wikipedia.org/wiki/Nucleobase"&gt;nucleobases&lt;/a&gt;&amp;nbsp;to&amp;nbsp;&lt;a href="https://en.wikipedia.org/wiki/Chromatogram"&gt;chromatogram&lt;/a&gt;&amp;nbsp;peaks), sequence alignment, trace visualization and variant detection.&lt;/p&gt;

&lt;p&gt;In this protocol, we describe our method used for analyzing sequence data obtained with our Applied Biosystems SeqStudio Genetic Analyzer instrument and analyzed using SnapGene software.&lt;/p&gt;</description>
  </descriptions>
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