May 7, 2020 Technical note Open Access

Nicouleau, Michael; Durcan, Thomas M.

DCAT Export

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        <foaf:name>Nicouleau, Michael</foaf:name>
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    <dct:title>Sequencing Analysis Guide</dct:title>
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    <dcat:keyword>sequencing</dcat:keyword>
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    <dct:description>&lt;p&gt;Sanger sequencing is a method of DNA sequencing that is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.&lt;/p&gt; &lt;p&gt;Sanger sequencing analysis software packages are used for base calling (process of assigning&amp;nbsp;&lt;a href="https://en.wikipedia.org/wiki/Nucleobase"&gt;nucleobases&lt;/a&gt;&amp;nbsp;to&amp;nbsp;&lt;a href="https://en.wikipedia.org/wiki/Chromatogram"&gt;chromatogram&lt;/a&gt;&amp;nbsp;peaks), sequence alignment, trace visualization and variant detection.&lt;/p&gt; &lt;p&gt;In this protocol, we describe our method used for analyzing sequence data obtained with our Applied Biosystems SeqStudio Genetic Analyzer instrument and analyzed using SnapGene software.&lt;/p&gt;</dct:description>
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