Technical note Open Access

Sequencing Analysis Guide

Nicouleau, Michael; Durcan, Thomas M.


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.3810825", 
  "language": "eng", 
  "title": "Sequencing Analysis Guide", 
  "issued": {
    "date-parts": [
      [
        2020, 
        5, 
        7
      ]
    ]
  }, 
  "abstract": "<p>Sanger sequencing is a method of DNA sequencing that is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.</p>\n\n<p>Sanger sequencing analysis software packages are used for base calling (process of assigning&nbsp;<a href=\"https://en.wikipedia.org/wiki/Nucleobase\">nucleobases</a>&nbsp;to&nbsp;<a href=\"https://en.wikipedia.org/wiki/Chromatogram\">chromatogram</a>&nbsp;peaks), sequence alignment, trace visualization and variant detection.</p>\n\n<p>In this protocol, we describe our method used for analyzing sequence data obtained with our Applied Biosystems SeqStudio Genetic Analyzer instrument and analyzed using SnapGene software.</p>", 
  "author": [
    {
      "family": "Nicouleau, Michael"
    }, 
    {
      "family": "Durcan, Thomas M."
    }
  ], 
  "version": "V1.0", 
  "type": "article", 
  "id": "3810825"
}
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