Massimo Ferrara, Antonio F. Logrieco, Antonio Moretti, Antonia Susca
2020-02-28
<p>Fumonisins contamination of food commodities is a worldwide problem, especially for maize. The ability to produce fumonisinsis a trait of several species of Fusarium, mainly F. verticillioides and F. proliferatum on maize, and some Aspergillus species. <em>A. niger</em> and its sister species <em>A. welwitschiae</em>, can contribute to fumonisin B<sub>2</sub> (FB<sub>2</sub>) accumulation in maize kernels, although to a lesser extent than fumonisin-producing <em>Fusarium</em> species.</p>
<p>Fumonisins risk monitoring represents an effective strategy in the integrated approach for mycotoxin risk management and reduction. The availability of a user-friendlymolecular assay for the detection oftoxigenic fungal species represents a valuable tool in understanding and managing upcoming mycotoxin contamination.</p>
<p>In this study, we developed a LAMP assay, based on the detection of <em>fum10</em>, for a rapid and specific molecular detection of FB<sub>2</sub>-producing <em>A. niger</em> and <em>A. welwistchiae</em>, potentially useful to perform monitoring directly “on site” in maize chain. Results showed that very low amounts of conidia are suitable to detect the presence of the target gene, thus providing information about the presence of FB<sub>2</sub>-producing <em>Aspergillus</em> species and the possible upcoming fumonisins contamination in maize. The assay was combined with a suitable protocol for “in field<em>”</em> crude DNA extraction and a colorimetric method for easy naked-eye evaluationof results, offering a reliable and user-friendly tool to support effective reduction strategies of mycotoxin contamination in crop management programs.</p>
https://doi.org/10.1016/j.fm.2020.103469
oai:zenodo.org:3752726
Zenodo
https://zenodo.org/communities/eu
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Aspergillus niger Aspergillus welwitschiae Rapid molecular tool Fumonisin Maize
A loop-mediated isothermal amplification (LAMP) assay for rapid detection of fumonisin producing Aspergillus species
info:eu-repo/semantics/article