3744912
doi
10.1016/j.algal.2019.101762
oai:zenodo.org:3744912
user-genialgproject
user-eu
Strittmatter, Martina
Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom ; Sorbonne Université, CNRS, ECOMAP team, UMR 7144, Adaptation and Diversity in the Marine Environment, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff, France
Jacquemin, Bertrand
CNRS, UMI 3614, Sorbonne Université, Pontifica Universidad Catolica de Chile, Universidad Austral ; Centre d'Etude et de Valorisation des Algues, Presqu'île de Pen Lan, 22610 Pleubian, France
Perrineau, Marie-Mathilde
Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom
Rousseau, Céline
PHENOTIC, SFR 4207 QUASAV, Angers, France
Badis, Yacine
Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom ; Sorbonne Université, CNRS, Algal Genetics Group, UMR 8227, Laboratory of Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff, France
Cock, J. Mark
Sorbonne Université, CNRS, Algal Genetics Group, UMR 8227, Laboratory of Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff, France
Destombe, Christophe
CNRS, UMI 3614, Sorbonne Université, Pontifica Universidad Catolica de Chile, Universidad Austral
Valero, Myriam
CNRS, UMI 3614, Sorbonne Université, Pontifica Universidad Catolica de Chile, Universidad Austral
Gachon, Claire M.M.
Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom ; Muséum National d'Histoire Naturelle, CNRS, UMR 7245 - Molécules de Communication et Adaptation des Micro-organismes , CP 54, 57 rue Cuvier, 75005 Paris, France
Parallelisable non-invasive biomass, fitness and growth measurement of macroalgae and other protists with nephelometry
Calmes, Benoit
Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom ; CNRS, UMI 3614, Sorbonne Université, Pontifica Universidad Catolica de Chile, Universidad Austral
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Biomass
Nephelometry
Algal cultivation
Biotechnology
Phenotyping
<p>With the exponential development of algal aquaculture and blue biotechnology, there is a strong demand for simple, inexpensive, high-throughput, quantitative phenotyping assays to measure the biomass, growth and fertility of algae and other marine protists. Here, we validate nephelometry, a method that relies on measuring the scattering of light by particles in suspension, as a non-invasive tool to measure in real-time the biomass of aquatic micro-organisms, such as microalgae, filamentous algae, as well as non-photosynthetic protists. Nephelometry is equally applicable to optic density and chlorophyll fluorescence measurements for the quantification of some microalgae, but outperforms other spectroscopy methods to quantify the biomass of biofilm-forming and filamentous algae, highly pigmented species and non-photosynthetic eukaryotes. Thanks to its insensitivity to the sample's pigmentation, nephelometry is also the method of choice when chlorophyll content varies between samples or time points, for example due to abiotic stress or pathogen infection. As examples, we illustrate how nephelometry can be combined with fluorometry or image analysis to monitor the quantity and time-course of spore release in fertile kelps or the progression of symptoms in diseased algal cultures.</p>
Zenodo
2020-01-31
info:eu-repo/semantics/article
3744911
user-genialgproject
user-eu
award_title=GENetic diversity exploitation for Innovative macro-ALGal biorefinery; award_number=727892; award_identifiers_scheme=url; award_identifiers_identifier=https://cordis.europa.eu/projects/727892; funder_id=00k4n6c32; funder_name=European Commission;
1586879947.441883
1010247
md5:d4b7d6793a877c1fdea4edb115ddb836
https://zenodo.org/records/3744912/files/Parallelisable non-invasive biomass, fitness and growth measurement of macroalgae and other protists with nephelometry.pdf
public