Journal article Open Access

Parallelisable non-invasive biomass, fitness and growth measurement of macroalgae and other protists with nephelometry

Calmes, Benoit; Strittmatter, Martina; Jacquemin, Bertrand; Perrineau, Marie-Mathilde; Rousseau, Céline; Badis, Yacine; Cock, J. Mark; Destombe, Christophe; Valero, Myriam; Gachon, Claire M.M.


MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="041" ind1=" " ind2=" ">
    <subfield code="a">eng</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Biomass</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Nephelometry</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Algal cultivation</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Biotechnology</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Phenotyping</subfield>
  </datafield>
  <controlfield tag="005">20200414155907.0</controlfield>
  <controlfield tag="001">3744912</controlfield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom ; Sorbonne  Université,  CNRS,  ECOMAP  team,  UMR  7144,  Adaptation  and  Diversity  in  the  Marine Environment, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff, France</subfield>
    <subfield code="a">Strittmatter, Martina</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">CNRS, UMI 3614, Sorbonne Université, Pontifica Universidad Catolica de Chile, Universidad Austral ; Centre d'Etude et de Valorisation des Algues, Presqu'île de Pen Lan,  22610 Pleubian, France</subfield>
    <subfield code="a">Jacquemin, Bertrand</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom</subfield>
    <subfield code="a">Perrineau, Marie-Mathilde</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">PHENOTIC, SFR 4207 QUASAV, Angers, France</subfield>
    <subfield code="a">Rousseau, Céline</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom ; Sorbonne Université, CNRS, Algal Genetics Group, UMR 8227, Laboratory of Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff, France</subfield>
    <subfield code="a">Badis, Yacine</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Sorbonne Université, CNRS, Algal Genetics Group, UMR 8227, Laboratory of Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff, France</subfield>
    <subfield code="a">Cock, J. Mark</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">CNRS, UMI 3614, Sorbonne Université, Pontifica Universidad Catolica de Chile, Universidad Austral</subfield>
    <subfield code="a">Destombe, Christophe</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">CNRS, UMI 3614, Sorbonne Université, Pontifica Universidad Catolica de Chile, Universidad Austral</subfield>
    <subfield code="a">Valero, Myriam</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom ; Muséum National d'Histoire Naturelle, CNRS, UMR 7245 - Molécules de Communication et Adaptation des Micro-organismes , CP 54, 57 rue Cuvier, 75005 Paris, France</subfield>
    <subfield code="a">Gachon, Claire M.M.</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">1010247</subfield>
    <subfield code="z">md5:d4b7d6793a877c1fdea4edb115ddb836</subfield>
    <subfield code="u">https://zenodo.org/record/3744912/files/Parallelisable non-invasive biomass, fitness and growth measurement of macroalgae and other protists with nephelometry.pdf</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2020-01-31</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire</subfield>
    <subfield code="p">user-genialgproject</subfield>
    <subfield code="o">oai:zenodo.org:3744912</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">Scottish Association for Marine Science, Scottish Marine Institute, PA37 1QA Oban, United Kingdom ;  CNRS, UMI 3614, Sorbonne Université, Pontifica Universidad Catolica de Chile, Universidad Austral</subfield>
    <subfield code="a">Calmes, Benoit</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Parallelisable non-invasive biomass, fitness and growth measurement of macroalgae and other protists with nephelometry</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-genialgproject</subfield>
  </datafield>
  <datafield tag="536" ind1=" " ind2=" ">
    <subfield code="c">727892</subfield>
    <subfield code="a">GENetic diversity exploitation for Innovative macro-ALGal biorefinery</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">https://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;With the exponential development of algal aquaculture and blue biotechnology, there is a strong demand for simple, inexpensive, high-throughput, quantitative phenotyping assays to measure the biomass, growth and fertility of algae and other marine protists. Here, we validate nephelometry, a method that relies on measuring the scattering of light by particles in suspension, as a non-invasive tool to measure in real-time the biomass of aquatic micro-organisms, such as microalgae, filamentous algae, as well as non-photosynthetic protists. Nephelometry is equally applicable to optic density and chlorophyll fluorescence measurements for the quantification of some microalgae, but outperforms other spectroscopy methods to quantify the biomass of biofilm-forming and filamentous algae, highly pigmented species and non-photosynthetic eukaryotes. Thanks to its insensitivity to the sample&amp;#39;s pigmentation, nephelometry is also the method of choice when chlorophyll content varies between samples or time points, for example due to abiotic stress or pathogen infection. As examples, we illustrate how nephelometry can be combined with fluorometry or image analysis to monitor the quantity and time-course of spore release in fertile kelps or the progression of symptoms in diseased algal cultures.&lt;/p&gt;</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.1016/j.algal.2019.101762</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">publication</subfield>
    <subfield code="b">article</subfield>
  </datafield>
</record>
45
41
views
downloads
Views 45
Downloads 41
Data volume 41.4 MB
Unique views 42
Unique downloads 40

Share

Cite as