Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology

Shlaifer, IrIna; Pimentel, Luisa; Sirois, Julien; Dorval, Genevieve; Beitel, Lenore; Durcan, Thomas M.


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  <dc:creator>Shlaifer, IrIna</dc:creator>
  <dc:creator>Pimentel, Luisa</dc:creator>
  <dc:creator>Sirois, Julien</dc:creator>
  <dc:creator>Dorval, Genevieve</dc:creator>
  <dc:creator>Beitel, Lenore</dc:creator>
  <dc:creator>Durcan, Thomas M.</dc:creator>
  <dc:date>2020-02-24</dc:date>
  <dc:description>This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.

Version 2.0 now available. </dc:description>
  <dc:identifier>https://zenodo.org/record/3738361</dc:identifier>
  <dc:identifier>10.5281/zenodo.3738361</dc:identifier>
  <dc:identifier>oai:zenodo.org:3738361</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>doi:10.5281/zenodo.3669098</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>https://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>CRISPR</dc:subject>
  <dc:subject>gene knockout</dc:subject>
  <dc:subject>genome editing</dc:subject>
  <dc:title>Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology</dc:title>
  <dc:type>info:eu-repo/semantics/technicalDocumentation</dc:type>
  <dc:type>publication-technicalnote</dc:type>
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