Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology

Shlaifer, IrIna; Pimentel, Luisa; Sirois, Julien; Dorval, Genevieve; Beitel, Lenore; Durcan, Thomas M.


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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p>This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.</p>\n\n<p>Version 2.0 now available.&nbsp;</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Shlaifer, IrIna"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Pimentel, Luisa"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Sirois, Julien"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Dorval, Genevieve"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Beitel, Lenore"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Durcan, Thomas M."
    }
  ], 
  "headline": "Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2020-02-24", 
  "url": "https://zenodo.org/record/3738361", 
  "version": "V2.0", 
  "keywords": [
    "CRISPR", 
    "gene knockout", 
    "genome editing"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.3738361", 
  "@id": "https://doi.org/10.5281/zenodo.3738361", 
  "@type": "ScholarlyArticle", 
  "name": "Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology"
}
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