Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology

Shlaifer, IrIna; Pimentel, Luisa; Sirois, Julien; Dorval, Genevieve; Beitel, Lenore; Durcan, Thomas M.


DCAT Export

<?xml version='1.0' encoding='utf-8'?>
<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:adms="http://www.w3.org/ns/adms#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dct="http://purl.org/dc/terms/" xmlns:dctype="http://purl.org/dc/dcmitype/" xmlns:dcat="http://www.w3.org/ns/dcat#" xmlns:duv="http://www.w3.org/ns/duv#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:frapo="http://purl.org/cerif/frapo/" xmlns:geo="http://www.w3.org/2003/01/geo/wgs84_pos#" xmlns:gsp="http://www.opengis.net/ont/geosparql#" xmlns:locn="http://www.w3.org/ns/locn#" xmlns:org="http://www.w3.org/ns/org#" xmlns:owl="http://www.w3.org/2002/07/owl#" xmlns:prov="http://www.w3.org/ns/prov#" xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" xmlns:schema="http://schema.org/" xmlns:skos="http://www.w3.org/2004/02/skos/core#" xmlns:vcard="http://www.w3.org/2006/vcard/ns#" xmlns:wdrs="http://www.w3.org/2007/05/powder-s#">
  <rdf:Description rdf:about="https://doi.org/10.5281/zenodo.3738361">
    <rdf:type rdf:resource="http://www.w3.org/ns/dcat#Dataset"/>
    <dct:type rdf:resource="http://purl.org/dc/dcmitype/Text"/>
    <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://doi.org/10.5281/zenodo.3738361</dct:identifier>
    <foaf:page rdf:resource="https://doi.org/10.5281/zenodo.3738361"/>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Shlaifer, IrIna</foaf:name>
        <foaf:givenName>IrIna</foaf:givenName>
        <foaf:familyName>Shlaifer</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>MNI, McGill University</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Pimentel, Luisa</foaf:name>
        <foaf:givenName>Luisa</foaf:givenName>
        <foaf:familyName>Pimentel</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>MNI, McGill University</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Sirois, Julien</foaf:name>
        <foaf:givenName>Julien</foaf:givenName>
        <foaf:familyName>Sirois</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>MNI, McGill University</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Dorval, Genevieve</foaf:name>
        <foaf:givenName>Genevieve</foaf:givenName>
        <foaf:familyName>Dorval</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>MNI, McGill University</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Beitel, Lenore</foaf:name>
        <foaf:givenName>Lenore</foaf:givenName>
        <foaf:familyName>Beitel</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>MNI, McGill University</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Durcan, Thomas M.</foaf:name>
        <foaf:givenName>Thomas M.</foaf:givenName>
        <foaf:familyName>Durcan</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>MNI, McGill University</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:title>Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology</dct:title>
    <dct:publisher>
      <foaf:Agent>
        <foaf:name>Zenodo</foaf:name>
      </foaf:Agent>
    </dct:publisher>
    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#gYear">2020</dct:issued>
    <dcat:keyword>CRISPR</dcat:keyword>
    <dcat:keyword>gene knockout</dcat:keyword>
    <dcat:keyword>genome editing</dcat:keyword>
    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#date">2020-02-24</dct:issued>
    <dct:language rdf:resource="http://publications.europa.eu/resource/authority/language/ENG"/>
    <owl:sameAs rdf:resource="https://zenodo.org/record/3738361"/>
    <adms:identifier>
      <adms:Identifier>
        <skos:notation rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://zenodo.org/record/3738361</skos:notation>
        <adms:schemeAgency>url</adms:schemeAgency>
      </adms:Identifier>
    </adms:identifier>
    <dct:isVersionOf rdf:resource="https://doi.org/10.5281/zenodo.3669098"/>
    <owl:versionInfo>V2.0</owl:versionInfo>
    <dct:description>&lt;p&gt;This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.&lt;/p&gt; &lt;p&gt;Version 2.0 now available.&amp;nbsp;&lt;/p&gt;</dct:description>
    <dct:accessRights rdf:resource="http://publications.europa.eu/resource/authority/access-right/PUBLIC"/>
    <dct:accessRights>
      <dct:RightsStatement rdf:about="info:eu-repo/semantics/openAccess">
        <rdfs:label>Open Access</rdfs:label>
      </dct:RightsStatement>
    </dct:accessRights>
    <dcat:distribution>
      <dcat:Distribution>
        <dct:license rdf:resource="https://creativecommons.org/licenses/by/4.0/legalcode"/>
        <dcat:accessURL rdf:resource="https://doi.org/10.5281/zenodo.3738361"/>
      </dcat:Distribution>
    </dcat:distribution>
    <dcat:distribution>
      <dcat:Distribution>
        <dcat:accessURL rdf:resource="https://doi.org/10.5281/zenodo.3738361"/>
        <dcat:byteSize>2682027</dcat:byteSize>
        <dcat:downloadURL rdf:resource="https://zenodo.org/record/3738361/files/EDDU-010-02_CRISPR gene KO_v2.0.pdf"/>
        <dcat:mediaType>application/pdf</dcat:mediaType>
      </dcat:Distribution>
    </dcat:distribution>
  </rdf:Description>
</rdf:RDF>
406
284
views
downloads
All versions This version
Views 406279
Downloads 284208
Data volume 774.4 MB557.9 MB
Unique views 325252
Unique downloads 262198

Share

Cite as