Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology

Shlaifer, IrIna; Pimentel, Luisa; Sirois, Julien; Dorval, Genevieve; Beitel, Lenore; Durcan, Thomas M.


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.3738361", 
  "language": "eng", 
  "title": "Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology", 
  "issued": {
    "date-parts": [
      [
        2020, 
        2, 
        24
      ]
    ]
  }, 
  "abstract": "<p>This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.</p>\n\n<p>Version 2.0 now available.&nbsp;</p>", 
  "author": [
    {
      "family": "Shlaifer, IrIna"
    }, 
    {
      "family": "Pimentel, Luisa"
    }, 
    {
      "family": "Sirois, Julien"
    }, 
    {
      "family": "Dorval, Genevieve"
    }, 
    {
      "family": "Beitel, Lenore"
    }, 
    {
      "family": "Durcan, Thomas M."
    }
  ], 
  "version": "V2.0", 
  "type": "article", 
  "id": "3738361"
}
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