Technical note Open Access

Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

Maneca, Destiny-Love; Luo, Wen; Krahn, Andrea; Del Cid Pellitero, Esther; Shlaifer, Irina; Beitel, Lenore K.; Rao, Trisha; Durcan, Thomas M.


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    <subfield code="a">&lt;p&gt;This protocol describes how to:&lt;br&gt;
&amp;bull; Produce and purify recombinant human and mouse &amp;alpha;-synuclein in monomeric form from transformed bacteria&lt;br&gt;
&amp;bull; Generate &amp;alpha;-synuclein preformed fibrils (PFFs) from recombinant monomeric &amp;alpha;-synuclein&lt;br&gt;
&amp;bull; Fluorescently label &amp;alpha;-synuclein PFFs&lt;/p&gt;

&lt;p&gt;Version 2.0 now available&lt;/p&gt;</subfield>
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