Technical note Open Access

Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

Maneca, Destiny-Love; Luo, Wen; Krahn, Andrea; Del Cid Pellitero, Esther; Shlaifer, Irina; Beitel, Lenore K.; Rao, Trisha; Durcan, Thomas M.


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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p>This protocol describes how to:<br>\n&bull; Produce and purify recombinant human and mouse &alpha;-synuclein in monomeric form from transformed bacteria<br>\n&bull; Generate &alpha;-synuclein preformed fibrils (PFFs) from recombinant monomeric &alpha;-synuclein<br>\n&bull; Fluorescently label &alpha;-synuclein PFFs</p>\n\n<p>Version 2.0 now available</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Maneca, Destiny-Love"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Luo, Wen"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Krahn, Andrea"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Del Cid Pellitero, Esther"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Shlaifer, Irina"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Beitel, Lenore K."
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Rao, Trisha"
    }, 
    {
      "affiliation": "MNI, McGill University", 
      "@type": "Person", 
      "name": "Durcan, Thomas M."
    }
  ], 
  "headline": "Production of Recombinant \u03b1 Synuclein Monomers and Preformed Fibrils (PFFs)", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2019-08-21", 
  "url": "https://zenodo.org/record/3738335", 
  "version": "V2.0", 
  "keywords": [
    "alpha-synuclein", 
    "protein expression", 
    "bacteria", 
    "GST-tag", 
    "Preformed fibrils (PFFs)"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.3738335", 
  "@id": "https://doi.org/10.5281/zenodo.3738335", 
  "@type": "ScholarlyArticle", 
  "name": "Production of Recombinant \u03b1 Synuclein Monomers and Preformed Fibrils (PFFs)"
}
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