Technical note Open Access

Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

Maneca, Destiny-Love; Luo, Wen; Krahn, Andrea; Del Cid Pellitero, Esther; Shlaifer, Irina; Beitel, Lenore K.; Rao, Trisha; Durcan, Thomas M.


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  <identifier identifierType="DOI">10.5281/zenodo.3738335</identifier>
  <creators>
    <creator>
      <creatorName>Maneca, Destiny-Love</creatorName>
      <givenName>Destiny-Love</givenName>
      <familyName>Maneca</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Luo, Wen</creatorName>
      <givenName>Wen</givenName>
      <familyName>Luo</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Krahn, Andrea</creatorName>
      <givenName>Andrea</givenName>
      <familyName>Krahn</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Del Cid Pellitero, Esther</creatorName>
      <givenName>Esther</givenName>
      <familyName>Del Cid Pellitero</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Shlaifer, Irina</creatorName>
      <givenName>Irina</givenName>
      <familyName>Shlaifer</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Beitel, Lenore K.</creatorName>
      <givenName>Lenore K.</givenName>
      <familyName>Beitel</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Rao, Trisha</creatorName>
      <givenName>Trisha</givenName>
      <familyName>Rao</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Durcan, Thomas M.</creatorName>
      <givenName>Thomas M.</givenName>
      <familyName>Durcan</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2019</publicationYear>
  <subjects>
    <subject>alpha-synuclein</subject>
    <subject>protein expression</subject>
    <subject>bacteria</subject>
    <subject>GST-tag</subject>
    <subject>Preformed fibrils (PFFs)</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2019-08-21</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Technical note</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/3738335</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3372887</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/sgc-opennotebook</relatedIdentifier>
  </relatedIdentifiers>
  <version>V2.0</version>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;This protocol describes how to:&lt;br&gt;
&amp;bull; Produce and purify recombinant human and mouse &amp;alpha;-synuclein in monomeric form from transformed bacteria&lt;br&gt;
&amp;bull; Generate &amp;alpha;-synuclein preformed fibrils (PFFs) from recombinant monomeric &amp;alpha;-synuclein&lt;br&gt;
&amp;bull; Fluorescently label &amp;alpha;-synuclein PFFs&lt;/p&gt;

&lt;p&gt;Version 2.0 now available&lt;/p&gt;</description>
  </descriptions>
</resource>
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