AGING AND CELLULAR MAGNETIC PROFILES DOCUMENTATION IN HAIR FOLLICLES CYCLES UTILIZING A NOVEL TABLETOP MAGNETIC PROFILES TECHNIQUE

Background: Recently, a tabletop optical microscopy technique was introduced to display living tissue magnetic profiles. The purpose of this manuscript is to introduce via the aforementioned technique in vitro experiments showing the effect of aging on tissue biophysical changes and documentation of cross species magnetic profiles. The term “metabolism” entails electron transfers involving movement of electrons from donor to acceptor, magnetic profiles are theorized to be a reflection of metabolic levels. Methods: The magnetic profiles technique calls for the completion of a blood smear on a 25x75x1mm glass slide. For this manuscript, the water in the original smear was allowed to evaporate; and a second smear was superimposed on the first. On the center surface of the top slide, freshly plucked scalp human hairs or plants and insects samples were processed. The preparations were then individually viewed microscopically and images recorded. Results: All living hair samples, ants and flower reproductive organs showed presence of magnetic profiles. Discussion: Images demonstrate that as tissue ages, (Experiments in Hair Follicles), there is a decrease in magnetic profiles (read metabolism), that these profiles display a chaotic interaction directly proportional with age. It could be inferred that the magnetic profiles experiments presented are equivalent to living tissue energy detection (read metabolism). The profiles technique could be applied to a wide range of cross species interactions. The term “metabolism” entails electron transfers involving movement of electrons from donor to acceptor along the electron transfer chain thus inducing magnetic profiles.


The magnetic Profiles Technique
The main purpose of this manuscript is to introduce the reader to the availability of an optical microscopy tabletop technique that displays living matter magnetic profiles, this includes animals, plants and also the interaction between animals and plants (1). A direct relationship between a prevalence of electromagnetism and energy emission from the hair dermal papilla had been previously demonstrated (2) and a number of publications have described a direct relationship between cellular aging and decrease in the basal metabolic rate (3). Historically a molecular approach has been used to evaluate metabolic changes (4,5).

In Vitro Experiments Aging and Cellular Energy
The hair follicle has been described as a miniorgan, with a variety of different cells having cellular divisions, and fluctuating metabolic process during growth cycles (6). Basically there is one main growth stage (Anagen), followed by a regressive (Catagen) and a resting one (Telogen) (Exhibit I).
Exhibit I: Hair Growth Cycles Reproduced from Shutterstock under the Fair Use policy. The purpose of this manuscript is to confirm via a biophysical method that detects magnetic profiles with previous molecular approach studies reports of residual metabolic activity in the hair follicle dermal papilla during the apoptosis and regress cycles. One of those studies contradict the historical notion of a total hair cycle shutdown or resting Telogen phase emphasized by the direct approach of the title "Resting no more: redefining Telogen…" (7). By using a molecular approach, experimental provocative maneuvers using a commonly used chemotherapy drug (cyclophosphamide) aimed to "induced follicle dystrophy and alopecia was used. In the experiments, massive keratinocyte apoptosis occurred in the entire distal hair follicle, except in the dermal papilla".

Material and Methods
The magnetic profiles technique calls for the preparation of a blood smear on a 25x75x1mm glass slide (8). For this manuscript, the water in the original smear was allowed to evaporate for 60±15 seconds; and a second evaporated smear was superimposed on the first (see note below). Both smears were then covered or sandwiched by a second similar glass slide. On the center surface of the top slide, a freshly plucked scalp human hairs on different growth ages (Anagen Catagen and Telogen) were placed and covered by two drops of distilled water. The preparations were then individually viewed microscopically and images recorded. The information downloaded to a computer photo application.
A detailed narrative describing the "Magnetic Profiles" technique could be found in the Materials and Methods Section of: http://www.jnsci.org/files/html/2016/e186.htm Technical Note: By having two superimposed single blood cells blood smears allows for a sharper visual qualitative delineation display of the magnetic profiles lines.

Results
All three human hair growth cycles expressed documented biomagnetic profiles. The initial growth stage (Anagen) showed evenly distributed magnetic profiles (

Discussion
The term "metabolism" entails electron transfers involving movement of electrons from donor to acceptor along the electron transfer chain thus inducing a current within each cell and from cell to cell, therefore and according to Faraday's Law and the Hall Effect, these currents induce electromagnetic fields (EMFs) perpendicular and horizontal, respectively, to the plane of the living tissues. Recently, a tabletop optical microscopy technique was introduced to display living tissue magnetic profiles. Http://www.granthaalayah.com ©International Journal of Research -GRANTHAALAYAH [248] As stated in the introduction, the main purpose of this manuscript is first to familiarize the reader with a novel slide assembly tabletop microscopy method designed to obtain magnetic profiles from living matter (Hair follicles in this case); and to demonstrate with this simple technique details of ie: The prevalence of metabolic activity (read magnetic profiles) in the no longer named resting Telogen hair follicle cycle as pointed by the large black arrow to the left of image in Figure 5 below, which had been previously molecularly demonstrated (9). Details of the technique can be obtained by linking to a paper that for the first time introduced the technique in the literature: http://www.jnsci.org/files/html/2016/e186.html

Magnetic Profiles Technique Explained from a Physicists Perspective
Results from the original experiments were of an unexplained nature. This author was surprised as to the fact that such a simple technique could produce images of magnetic profiles from living tissue; therefore hair follicles were immersed in 35% H2O2 to induce a total depletion of the enzyme catalase. In a separate publication, the technique was challenged and validated by provocative maneuvers designed to document the lack of magnetic profiles in dead follicles (10) (Figure S1 below).

Seeking Professional Opinion
This biologist needed help; and the images were sent for interpretation to a renowned physicist. His written reply is on file and reproduced in Exhibit I below: Cyclotron Once the technique is mastered, the reader could explore the until now less traveled road of displaying living matter magnetic profiles. Below are examples of the potential experimental range seen in mages that could be obtained from plants, insects or cross-species interactions ( Figures S2  thru S4).

Possible Medical Applications
The effect of medication therapy on the hair follicle metabolism could now be easily evaluated. Information as to the progress of diseases such as Alopecia is now a possibility (11). The question arises: How are magnetic profiles (read metabolism) expressed in the progression of Alopecia?
…or: Could this technique aid in the diagnosis and treatment in rare skin/hair human diseases such as Confluent Reticulated Papillomatosis (CRP)? (12). Further research is warranted.