Journal article Open Access

PNA-Based Dynamic Combinatorial libraries (PDCL) and screening of lectins

Lluc Farrera-Soler; Jean-Pierre Daguer; Patrick Raunft; Anne Imberty; Sofia Barluenga; Nicolas Winssinger


JSON Export

{
  "files": [
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Biotinyation%20RSL.txt"
      }, 
      "checksum": "md5:65e3a5898c951336158d9f756a2625e4", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Biotinyation RSL.txt", 
      "type": "txt", 
      "size": 2143255
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Biotinylation%20AFL.txt"
      }, 
      "checksum": "md5:4a49fe5d271fd5d2fbcbcf4d8af4d1f9", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Biotinylation AFL.txt", 
      "type": "txt", 
      "size": 6215177
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Full%20library%20starting%20library.txt"
      }, 
      "checksum": "md5:309f85b044eb59b12c469c5f979dfc69", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Full library starting library.txt", 
      "type": "txt", 
      "size": 257653
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Full%20selection%20RSL%20part%201.txt"
      }, 
      "checksum": "md5:5bfc920f17e57f94813c73f167157332", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Full selection RSL part 1.txt", 
      "type": "txt", 
      "size": 35458
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Full%20selection%20RSL%20part%202.txt"
      }, 
      "checksum": "md5:e75ef69ff885e7ff4e9b7fb5b3d3cd91", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Full selection RSL part 2.txt", 
      "type": "txt", 
      "size": 32482
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Full%20selection%20RSL%20part%203.txt"
      }, 
      "checksum": "md5:a268369fc6bf9d13a61d64355874b495", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Full selection RSL part 3.txt", 
      "type": "txt", 
      "size": 32062
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Full%20selection%20RSL%20part%204.txt"
      }, 
      "checksum": "md5:03a86419c5bdd07fa33608136061a4e3", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Full selection RSL part 4.txt", 
      "type": "txt", 
      "size": 31218
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/FUll%20selection%20RSL%20part%205"
      }, 
      "checksum": "md5:643bffd7f59523203aac4d904e0f596d", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "FUll selection RSL part 5", 
      "type": "", 
      "size": 14680
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/M0A.txt"
      }, 
      "checksum": "md5:fb5429a0e7f0e356b83f043b5333900e", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "M0A.txt", 
      "type": "txt", 
      "size": 2887817
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/MKA.txt"
      }, 
      "checksum": "md5:44a29efaaea8dd6f46b68563f5523bc7", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "MKA.txt", 
      "type": "txt", 
      "size": 2709259
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/MXA.txt"
      }, 
      "checksum": "md5:c32405232594792dfb53b903ba189a23", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "MXA.txt", 
      "type": "txt", 
      "size": 1831143
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/N0A.txt"
      }, 
      "checksum": "md5:cd9f753bedebe00dc0201e3e4c5c5c8f", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "N0A.txt", 
      "type": "txt", 
      "size": 2468454
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/NKA.txt"
      }, 
      "checksum": "md5:74f425dc2971a7300234eeea7540b914", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "NKA.txt", 
      "type": "txt", 
      "size": 2700792
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/No%20protein%20fucose%20glucose%201%20to%201.txt"
      }, 
      "checksum": "md5:a1a477d6f77304c31679232810595094", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "No protein fucose glucose 1 to 1.txt", 
      "type": "txt", 
      "size": 38753
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/no%20protein%20fucose%20glucose%201%20to%2099.txt"
      }, 
      "checksum": "md5:a1a477d6f77304c31679232810595094", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "no protein fucose glucose 1 to 99.txt", 
      "type": "txt", 
      "size": 38753
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/NXA.txt"
      }, 
      "checksum": "md5:c32405232594792dfb53b903ba189a23", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "NXA.txt", 
      "type": "txt", 
      "size": 1831143
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/RSL%20selection%20big%20library%2060C.txt"
      }, 
      "checksum": "md5:972757b1915dbb2981a92c13670027c9", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "RSL selection big library 60C.txt", 
      "type": "txt", 
      "size": 111603
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Selection%20AFL%20rt.txt"
      }, 
      "checksum": "md5:9c169a1fb85e5e2e91d85d88f5770439", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Selection AFL rt.txt", 
      "type": "txt", 
      "size": 198054
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Selection%20full%20library%20RSL%20rt.txt"
      }, 
      "checksum": "md5:65e441ff2ff50b0e99e6d015243d548a", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Selection full library RSL rt.txt", 
      "type": "txt", 
      "size": 237627
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Selection%20RSL%201%20to%201.txt"
      }, 
      "checksum": "md5:c87f40653c65da8944e9c32986677e29", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Selection RSL 1 to 1.txt", 
      "type": "txt", 
      "size": 37858
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Selection%20RSL%20fucose%20glucose%201%20to%2099.txt"
      }, 
      "checksum": "md5:0c20b1e69f06ef9e02972469425be9be", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Selection RSL fucose glucose 1 to 99.txt", 
      "type": "txt", 
      "size": 37868
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Single%20sequence%20Fucose.txt"
      }, 
      "checksum": "md5:4fdc04c7fe9dbce8129897590a16075e", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Single sequence Fucose.txt", 
      "type": "txt", 
      "size": 2295349
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Single%20sequence%20glucose.txt"
      }, 
      "checksum": "md5:b749a80ad2ade6f96cd93ffd48c61656", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Single sequence glucose.txt", 
      "type": "txt", 
      "size": 3022906
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/SPR_AFL_2fucosyllactose_alone"
      }, 
      "checksum": "md5:ef1f98bf35b465052a486e457a5b31a6", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "SPR_AFL_2fucosyllactose_alone", 
      "type": "", 
      "size": 706560
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/SPR_AFL_Hits"
      }, 
      "checksum": "md5:7f2dddd4d19837515ca1ee90ded56484", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "SPR_AFL_Hits", 
      "type": "", 
      "size": 29592064
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/SPR_MOA_MXA_MKA_alone_with_AFL"
      }, 
      "checksum": "md5:14d44520c438c3bba418bd0879ff1aa7", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "SPR_MOA_MXA_MKA_alone_with_AFL", 
      "type": "", 
      "size": 7863296
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/SPR_MOA_MXA_MKA_alone_with_RSL"
      }, 
      "checksum": "md5:0bf8dcb5eeb9a0a506b08852bc3ff5a0", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "SPR_MOA_MXA_MKA_alone_with_RSL", 
      "type": "", 
      "size": 7863296
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/SPR_RSL_2fucosyllactosealone"
      }, 
      "checksum": "md5:7bf060fe85964dd439655fe79e63b284", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "SPR_RSL_2fucosyllactosealone", 
      "type": "", 
      "size": 755200
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/SPR_RSL_Hits"
      }, 
      "checksum": "md5:de51afbe1e6f744b1fa253484e0222fe", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "SPR_RSL_Hits", 
      "type": "", 
      "size": 29585920
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/starting%20lib%202fucosyllactose.txt"
      }, 
      "checksum": "md5:300ccaac44f56a11ee12c061c6e600ce", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "starting lib 2fucosyllactose.txt", 
      "type": "txt", 
      "size": 290634
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20lib%20cellobiose.txt"
      }, 
      "checksum": "md5:829b5f479c0cecd04a421293bdf9b13f", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting lib cellobiose.txt", 
      "type": "txt", 
      "size": 297576
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20lib%20GalSPhe.txt"
      }, 
      "checksum": "md5:7710727b43e0ca103eeb956d3e29783c", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting lib GalSPhe.txt", 
      "type": "txt", 
      "size": 284399
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20lib%20GlcNAc%20cluster.txt"
      }, 
      "checksum": "md5:8bf2879303d88f5877c4138d38d68691", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting lib GlcNAc cluster.txt", 
      "type": "txt", 
      "size": 256424
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20lib%20glucoseamine.txt"
      }, 
      "checksum": "md5:e804f1d226b08d9a5f11555a42a49ca9", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting lib glucoseamine.txt", 
      "type": "txt", 
      "size": 204622
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20lib%20glucose.txt"
      }, 
      "checksum": "md5:0da16ce3d8b25db246ed151e0e55e3c2", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting lib glucose.txt", 
      "type": "txt", 
      "size": 299117
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20lib%20GluNAc.txt"
      }, 
      "checksum": "md5:c7d1cd8afd5dfd48a122edb7f1cd9edb", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting lib GluNAc.txt", 
      "type": "txt", 
      "size": 294688
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20lib%20Maltose.txt"
      }, 
      "checksum": "md5:258534aacdb663fe451545c7b688044f", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting lib Maltose.txt", 
      "type": "txt", 
      "size": 295385
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20lib%20Mannose.txt"
      }, 
      "checksum": "md5:291796dc462826fe69d9e490afd58121", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting lib Mannose.txt", 
      "type": "txt", 
      "size": 279765
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20library%201%20to%201%20fucose%20glucose.txt"
      }, 
      "checksum": "md5:b77c7ac652eaf04fffc3cc8c42bc351b", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting library 1 to 1 fucose glucose.txt", 
      "type": "txt", 
      "size": 45491
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20library%20fucose%20glucose%201%20to%2099.txt"
      }, 
      "checksum": "md5:577e0a1d276f806a362e75e88df84727", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting library fucose glucose 1 to 99.txt", 
      "type": "txt", 
      "size": 37191
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf/Starting%20lib%20xylose.txt"
      }, 
      "checksum": "md5:52a8e81293ad5f2e6fed104b627092fb", 
      "bucket": "2c103c70-8580-415d-983d-3ad547d54caf", 
      "key": "Starting lib xylose.txt", 
      "type": "txt", 
      "size": 285332
    }
  ], 
  "owners": [
    94663
  ], 
  "doi": "10.5281/zenodo.3712677", 
  "stats": {
    "version_unique_downloads": 9.0, 
    "unique_views": 25.0, 
    "views": 27.0, 
    "version_views": 27.0, 
    "unique_downloads": 9.0, 
    "version_unique_views": 25.0, 
    "volume": 34946903.0, 
    "version_downloads": 10.0, 
    "downloads": 10.0, 
    "version_volume": 34946903.0
  }, 
  "links": {
    "doi": "https://doi.org/10.5281/zenodo.3712677", 
    "conceptdoi": "https://doi.org/10.5281/zenodo.3712676", 
    "bucket": "https://zenodo.org/api/files/2c103c70-8580-415d-983d-3ad547d54caf", 
    "conceptbadge": "https://zenodo.org/badge/doi/10.5281/zenodo.3712676.svg", 
    "html": "https://zenodo.org/record/3712677", 
    "latest_html": "https://zenodo.org/record/3712677", 
    "badge": "https://zenodo.org/badge/doi/10.5281/zenodo.3712677.svg", 
    "latest": "https://zenodo.org/api/records/3712677"
  }, 
  "conceptdoi": "10.5281/zenodo.3712676", 
  "created": "2020-03-26T20:51:57.323584+00:00", 
  "updated": "2020-09-24T11:38:12.126888+00:00", 
  "conceptrecid": "3712676", 
  "revision": 3, 
  "id": 3712677, 
  "metadata": {
    "access_right_category": "success", 
    "doi": "10.5281/zenodo.3712677", 
    "description": "<p>Selection from dynamic combinatorial libraries (DCL) benefit from the dynamic nature of the library that can change constitution upon addition of a selection pressure, such as ligands binding to a protein. This technology has been predominantly used with small molecules interacting with each other through reversible covalent interaction. However, application of this technology in biomedical research and drug discovery has been limited by the reversibility of covalent exchange and the analytical deconvolution of small molecule fragments.&nbsp;&nbsp; Here we report a supramolecular approach based on the use of a constant short PNA tag to direct the combinatorial pairing of fragment.&nbsp; This PNA tag yields fast exchange kinetics, while still delivering the benefits of cooperativity, and provides favourable properties for analytical deconvolution by MALDI.&nbsp; A selection of &gt; 6 000 assemblies of glycans (mono-, di-, tri-saccharides) targeting AFL, a lectin from pathogenic fungus, yielded a 95 nM assembly, nearly three orders of magnitude better affinity than the corresponding glycan alone (41 &micro;M).</p>", 
    "license": {
      "id": "CC-BY-4.0"
    }, 
    "title": "PNA-Based Dynamic Combinatorial libraries (PDCL) and screening of lectins", 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "3712676"
          }, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "3712677"
          }
        }
      ]
    }, 
    "grants": [
      {
        "code": "200020_169141", 
        "links": {
          "self": "https://zenodo.org/api/grants/10.13039/501100001711::200020_169141"
        }, 
        "title": "Probes for chemical biology: from synthesis to biosupramolecular systems", 
        "acronym": "", 
        "program": "Project funding", 
        "funder": {
          "doi": "10.13039/501100001711", 
          "acronyms": [], 
          "name": "Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung", 
          "links": {
            "self": "https://zenodo.org/api/funders/10.13039/501100001711"
          }
        }
      }
    ], 
    "publication_date": "2020-03-17", 
    "creators": [
      {
        "affiliation": "University of Geneva", 
        "name": "Lluc Farrera-Soler"
      }, 
      {
        "affiliation": "University of Geneva", 
        "name": "Jean-Pierre Daguer"
      }, 
      {
        "affiliation": "University of Geneva", 
        "name": "Patrick Raunft"
      }, 
      {
        "orcid": "0000-0001-6825-9527", 
        "affiliation": "Universit\u00e9 Grenoble Alpes", 
        "name": "Anne Imberty"
      }, 
      {
        "orcid": "0000-0003-3830-1178", 
        "affiliation": "University of Geneva", 
        "name": "Sofia Barluenga"
      }, 
      {
        "orcid": "0000-0003-1636-7766", 
        "affiliation": "University of Geneva", 
        "name": "Nicolas Winssinger"
      }
    ], 
    "access_right": "open", 
    "resource_type": {
      "subtype": "article", 
      "type": "publication", 
      "title": "Journal article"
    }, 
    "related_identifiers": [
      {
        "scheme": "doi", 
        "identifier": "10.5281/zenodo.3712676", 
        "relation": "isVersionOf"
      }
    ]
  }
}
27
10
views
downloads
All versions This version
Views 2727
Downloads 1010
Data volume 34.9 MB34.9 MB
Unique views 2525
Unique downloads 99

Share

Cite as