Journal article Open Access
PNA-Based Dynamic Combinatorial libraries (PDCL) and screening of lectins
Lluc Farrera-Soler;
Jean-Pierre Daguer;
Patrick Raunft;
Anne Imberty;
Sofia Barluenga;
Nicolas Winssinger
Selection from dynamic combinatorial libraries (DCL) benefit from the dynamic nature of the library that can change constitution upon addition of a selection pressure, such as ligands binding to a protein. This technology has been predominantly used with small molecules interacting with each other through reversible covalent interaction. However, application of this technology in biomedical research and drug discovery has been limited by the reversibility of covalent exchange and the analytical deconvolution of small molecule fragments. Here we report a supramolecular approach based on the use of a constant short PNA tag to direct the combinatorial pairing of fragment. This PNA tag yields fast exchange kinetics, while still delivering the benefits of cooperativity, and provides favourable properties for analytical deconvolution by MALDI. A selection of > 6 000 assemblies of glycans (mono-, di-, tri-saccharides) targeting AFL, a lectin from pathogenic fungus, yielded a 95 nM assembly, nearly three orders of magnitude better affinity than the corresponding glycan alone (41 µM).
| Name | Size | |
|---|---|---|
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Biotinyation RSL.txt
md5:65e3a5898c951336158d9f756a2625e4 |
2.1 MB | Download |
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Biotinylation AFL.txt
md5:4a49fe5d271fd5d2fbcbcf4d8af4d1f9 |
6.2 MB | Download |
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Full library starting library.txt
md5:309f85b044eb59b12c469c5f979dfc69 |
257.7 kB | Download |
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Full selection RSL part 1.txt
md5:5bfc920f17e57f94813c73f167157332 |
35.5 kB | Download |
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Full selection RSL part 2.txt
md5:e75ef69ff885e7ff4e9b7fb5b3d3cd91 |
32.5 kB | Download |
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Full selection RSL part 3.txt
md5:a268369fc6bf9d13a61d64355874b495 |
32.1 kB | Download |
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Full selection RSL part 4.txt
md5:03a86419c5bdd07fa33608136061a4e3 |
31.2 kB | Download |
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FUll selection RSL part 5
md5:643bffd7f59523203aac4d904e0f596d |
14.7 kB | Download |
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M0A.txt
md5:fb5429a0e7f0e356b83f043b5333900e |
2.9 MB | Download |
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MKA.txt
md5:44a29efaaea8dd6f46b68563f5523bc7 |
2.7 MB | Download |
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MXA.txt
md5:c32405232594792dfb53b903ba189a23 |
1.8 MB | Download |
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N0A.txt
md5:cd9f753bedebe00dc0201e3e4c5c5c8f |
2.5 MB | Download |
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NKA.txt
md5:74f425dc2971a7300234eeea7540b914 |
2.7 MB | Download |
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No protein fucose glucose 1 to 1.txt
md5:a1a477d6f77304c31679232810595094 |
38.8 kB | Download |
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no protein fucose glucose 1 to 99.txt
md5:a1a477d6f77304c31679232810595094 |
38.8 kB | Download |
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NXA.txt
md5:c32405232594792dfb53b903ba189a23 |
1.8 MB | Download |
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RSL selection big library 60C.txt
md5:972757b1915dbb2981a92c13670027c9 |
111.6 kB | Download |
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Selection AFL rt.txt
md5:9c169a1fb85e5e2e91d85d88f5770439 |
198.1 kB | Download |
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Selection full library RSL rt.txt
md5:65e441ff2ff50b0e99e6d015243d548a |
237.6 kB | Download |
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Selection RSL 1 to 1.txt
md5:c87f40653c65da8944e9c32986677e29 |
37.9 kB | Download |
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Selection RSL fucose glucose 1 to 99.txt
md5:0c20b1e69f06ef9e02972469425be9be |
37.9 kB | Download |
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Single sequence Fucose.txt
md5:4fdc04c7fe9dbce8129897590a16075e |
2.3 MB | Download |
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Single sequence glucose.txt
md5:b749a80ad2ade6f96cd93ffd48c61656 |
3.0 MB | Download |
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SPR_AFL_2fucosyllactose_alone
md5:ef1f98bf35b465052a486e457a5b31a6 |
706.6 kB | Download |
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SPR_AFL_Hits
md5:7f2dddd4d19837515ca1ee90ded56484 |
29.6 MB | Download |
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SPR_MOA_MXA_MKA_alone_with_AFL
md5:14d44520c438c3bba418bd0879ff1aa7 |
7.9 MB | Download |
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SPR_MOA_MXA_MKA_alone_with_RSL
md5:0bf8dcb5eeb9a0a506b08852bc3ff5a0 |
7.9 MB | Download |
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SPR_RSL_2fucosyllactosealone
md5:7bf060fe85964dd439655fe79e63b284 |
755.2 kB | Download |
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SPR_RSL_Hits
md5:de51afbe1e6f744b1fa253484e0222fe |
29.6 MB | Download |
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starting lib 2fucosyllactose.txt
md5:300ccaac44f56a11ee12c061c6e600ce |
290.6 kB | Download |
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Starting lib cellobiose.txt
md5:829b5f479c0cecd04a421293bdf9b13f |
297.6 kB | Download |
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Starting lib GalSPhe.txt
md5:7710727b43e0ca103eeb956d3e29783c |
284.4 kB | Download |
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Starting lib GlcNAc cluster.txt
md5:8bf2879303d88f5877c4138d38d68691 |
256.4 kB | Download |
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Starting lib glucose.txt
md5:0da16ce3d8b25db246ed151e0e55e3c2 |
299.1 kB | Download |
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Starting lib glucoseamine.txt
md5:e804f1d226b08d9a5f11555a42a49ca9 |
204.6 kB | Download |
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Starting lib GluNAc.txt
md5:c7d1cd8afd5dfd48a122edb7f1cd9edb |
294.7 kB | Download |
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Starting lib Maltose.txt
md5:258534aacdb663fe451545c7b688044f |
295.4 kB | Download |
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Starting lib Mannose.txt
md5:291796dc462826fe69d9e490afd58121 |
279.8 kB | Download |
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Starting lib xylose.txt
md5:52a8e81293ad5f2e6fed104b627092fb |
285.3 kB | Download |
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Starting library 1 to 1 fucose glucose.txt
md5:b77c7ac652eaf04fffc3cc8c42bc351b |
45.5 kB | Download |
|
Starting library fucose glucose 1 to 99.txt
md5:577e0a1d276f806a362e75e88df84727 |
37.2 kB | Download |
| All versions | This version | |
|---|---|---|
| Views | 66 | 66 |
| Downloads | 67 | 67 |
| Data volume | 214.9 MB | 214.9 MB |
| Unique views | 62 | 62 |
| Unique downloads | 60 | 60 |
- Publication date:
- March 17, 2020
- DOI:
-
- Grants:
-
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung:
- Probes for chemical biology: from synthesis to biosupramolecular systems (200020_169141)
- License (for files):
- Creative Commons Attribution 4.0 International