Software Open Access

Cell Fluorescence Quantification

Stéphane Rigaud; Jessica Quintin


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    <subfield code="a">&lt;p&gt;ICY software batch protocol for automatic cell fluorescence measurement in cell culture images.&lt;/p&gt;

&lt;p&gt;The automatically identify the cell from the background using an HK-means approach and measure both area and intensity inside each cells before saving it into an excel sheet for analysis.&lt;/p&gt;

&lt;p&gt;This protocol was used for the potassium intensity measurement in cells under fluorescence binocular.&lt;/p&gt;

&lt;p&gt;This work was funded by the following:&lt;/p&gt;

&lt;ul&gt;
	&lt;li&gt;ANR JCJC grant (ANR-16-CE15-0014-01).&lt;/li&gt;
	&lt;li&gt;Institut Carnot&amp;ndash;Microbes et Sant&amp;eacute; grant (n&amp;deg;11 CARN-017-01).&lt;/li&gt;
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