Software Open Access

Cell Fluorescence Quantification

Stéphane Rigaud; Jessica Quintin


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        <foaf:name>Jessica Quintin</foaf:name>
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    <dct:title>Cell Fluorescence Quantification</dct:title>
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    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#gYear">2020</dct:issued>
    <dcat:keyword>ICY</dcat:keyword>
    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#date">2020-03-11</dct:issued>
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    <dct:description>&lt;p&gt;ICY software batch protocol for automatic cell fluorescence measurement in cell culture images.&lt;/p&gt; &lt;p&gt;The automatically identify the cell from the background using an HK-means approach and measure both area and intensity inside each cells before saving it into an excel sheet for analysis.&lt;/p&gt; &lt;p&gt;This protocol was used for the potassium intensity measurement in cells under fluorescence binocular.&lt;/p&gt; &lt;p&gt;This work was funded by the following:&lt;/p&gt; &lt;ul&gt; &lt;li&gt;ANR JCJC grant (ANR-16-CE15-0014-01).&lt;/li&gt; &lt;li&gt;Institut Carnot&amp;ndash;Microbes et Sant&amp;eacute; grant (n&amp;deg;11 CARN-017-01).&lt;/li&gt; &lt;/ul&gt;</dct:description>
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