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Published February 24, 2020 | Version V1.0
Technical note Open

CRISPR-mediated introduction of single nucleotide changes in iPSCs

Creators

  • 1. MNI, McGill University

Description

This protocol describes how to edit an individual nucleotide in the genome of human induced pluripotent stem cells (iPSCs), combining CRISPR-Cas9 editing, droplet digital PCR (ddPCR) technology and antibiotic-free selection.

Files

CRISPR Cas9 ddPCR SOP v1.0_merged.pdf

Files (1.2 MB)

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