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Technical note Open Access

Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology

Shlaifer, IrIna; Pimentel, Luisa; Sirois, Julien; Dorval, Genevieve; Beitel, Lenore; Durcan, Thomas M.

This protocol describes a CRISPR-Cas9 genome editing method to generate knockout (KO) cell lines.

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