Dataset Open Access

Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles

Mahecic, Dora; Gambarotto, Davide; Douglass, Kyle M.; Fortun, Denis; Banterle, Niccoló; Le Guennec, Maeva; Ibrahim, Khalid; Gönczy, Pierre; Hamel, Virginie; Guichard, Paul; Manley, Suliana


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    <subfield code="a">&lt;p&gt;Datasets containing the segmented expanded centriole particles. The prefix Hs is used to denote particles acquired in synchronized RPE-1 human cells. Otherwise particles were collected from expanded isolated centrioles from &lt;em&gt;Chlamydomoanas reinhardtii&lt;/em&gt;. Resized datasets have uniform voxel size of 14x14x14 nm3 after expansion (56x56x56 nm3 before expansion). Non-resized datasets have 14x14x30 pixel size (56x56x120 nm3 before expansion). All files should be mirrored horizontally/vertically to account for the chirality inversion due to the imaging process&lt;/p&gt;

&lt;p&gt;The channels in different datasets are:&lt;/p&gt;

&lt;ul&gt;
	&lt;li&gt;Chlamy acetylated sample:
	&lt;ul&gt;
		&lt;li&gt;C1: acetylated tubulin-Alexa488&lt;/li&gt;
		&lt;li&gt;C2: aTubulin-Alexa568&lt;/li&gt;
	&lt;/ul&gt;
	&lt;/li&gt;
	&lt;li&gt;Chlamy MonoE sample
	&lt;ul&gt;
		&lt;li&gt;C1: aTubulin-Alexa488&lt;/li&gt;
		&lt;li&gt;C2: GT335-Alexa568&lt;/li&gt;
	&lt;/ul&gt;
	&lt;/li&gt;
	&lt;li&gt;Chlamy PolyE sample
	&lt;ul&gt;
		&lt;li&gt;C1: PolyE-Alexa488&lt;/li&gt;
		&lt;li&gt;C2: aTubulin-Alexa568&lt;/li&gt;
	&lt;/ul&gt;
	&lt;/li&gt;
	&lt;li&gt;Hs sample:
	&lt;ul&gt;
		&lt;li&gt;C1: PolyE-Alexa488&lt;/li&gt;
		&lt;li&gt;C2: acetylated tubulin-Alexa586&lt;/li&gt;
	&lt;/ul&gt;
	&lt;/li&gt;
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