Dataset Open Access

Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles

Mahecic, Dora; Gambarotto, Davide; Douglass, Kyle M.; Fortun, Denis; Banterle, Niccoló; Le Guennec, Maeva; Ibrahim, Khalid; Gönczy, Pierre; Hamel, Virginie; Guichard, Paul; Manley, Suliana

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<oai_dc:dc xmlns:dc="" xmlns:oai_dc="" xmlns:xsi="" xsi:schemaLocation="">
  <dc:creator>Mahecic, Dora</dc:creator>
  <dc:creator>Gambarotto, Davide</dc:creator>
  <dc:creator>Douglass, Kyle M.</dc:creator>
  <dc:creator>Fortun, Denis</dc:creator>
  <dc:creator>Banterle, Niccoló</dc:creator>
  <dc:creator>Le Guennec, Maeva</dc:creator>
  <dc:creator>Ibrahim, Khalid</dc:creator>
  <dc:creator>Gönczy, Pierre</dc:creator>
  <dc:creator>Hamel, Virginie</dc:creator>
  <dc:creator>Guichard, Paul</dc:creator>
  <dc:creator>Manley, Suliana</dc:creator>
  <dc:description>Datasets containing the segmented expanded centriole particles. The prefix Hs is used to denote particles acquired in synchronized RPE-1 human cells. Otherwise particles were collected from expanded isolated centrioles from Chlamydomoanas reinhardtii. Resized datasets have uniform voxel size of 14x14x14 nm3 after expansion (56x56x56 nm3 before expansion). Non-resized datasets have 14x14x30 pixel size (56x56x120 nm3 before expansion). All files should be mirrored horizontally/vertically to account for the chirality inversion due to the imaging process

The channels in different datasets are:

	Chlamy acetylated sample:
		C1: acetylated tubulin-Alexa488
		C2: aTubulin-Alexa568
	Chlamy MonoE sample
		C1: aTubulin-Alexa488
		C2: GT335-Alexa568
	Chlamy PolyE sample
		C1: PolyE-Alexa488
		C2: aTubulin-Alexa568
	Hs sample:
		C1: PolyE-Alexa488
		C2: acetylated tubulin-Alexa586
  <dc:subject>ultrastructure expansion microscopy; iSIM;</dc:subject>
  <dc:title>Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles</dc:title>
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