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Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles

Mahecic, Dora; Gambarotto, Davide; Douglass, Kyle M.; Fortun, Denis; Banterle, Niccoló; Le Guennec, Maeva; Ibrahim, Khalid; Gönczy, Pierre; Hamel, Virginie; Guichard, Paul; Manley, Suliana


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  <identifier identifierType="DOI">10.5281/zenodo.3613906</identifier>
  <creators>
    <creator>
      <creatorName>Mahecic, Dora</creatorName>
      <givenName>Dora</givenName>
      <familyName>Mahecic</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-1004-4771</nameIdentifier>
      <affiliation>Laboratory for Experimental Biophysics, Institute of Physics, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, SwitzerlandSwiss National Centre for Competence in Research (NCCR) in Chemical Biology, University of Geneva, Geneva, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Gambarotto, Davide</creatorName>
      <givenName>Davide</givenName>
      <familyName>Gambarotto</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-4691-082X</nameIdentifier>
      <affiliation>Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Douglass, Kyle M.</creatorName>
      <givenName>Kyle M.</givenName>
      <familyName>Douglass</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-4694-0922</nameIdentifier>
      <affiliation>Laboratory for Experimental Biophysics, Institute of Physics, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Fortun, Denis</creatorName>
      <givenName>Denis</givenName>
      <familyName>Fortun</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-1275-4697</nameIdentifier>
      <affiliation>ICube, CNRS, University of Strasbourg, Illkirch, France</affiliation>
    </creator>
    <creator>
      <creatorName>Banterle, Niccoló</creatorName>
      <givenName>Niccoló</givenName>
      <familyName>Banterle</familyName>
      <affiliation>Swiss National Centre for Competence in Research (NCCR) in Chemical Biology, University of Geneva, Geneva, SwitzerlandSwiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Le Guennec, Maeva</creatorName>
      <givenName>Maeva</givenName>
      <familyName>Le Guennec</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-3789-7988</nameIdentifier>
      <affiliation>Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Ibrahim, Khalid</creatorName>
      <givenName>Khalid</givenName>
      <familyName>Ibrahim</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-1819-1952</nameIdentifier>
      <affiliation>Laboratory for Experimental Biophysics, Institute of Physics, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Gönczy, Pierre</creatorName>
      <givenName>Pierre</givenName>
      <familyName>Gönczy</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-6305-6883</nameIdentifier>
      <affiliation>Swiss National Centre for Competence in Research (NCCR) in Chemical Biology, University of Geneva, Geneva, SwitzerlandSwiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Hamel, Virginie</creatorName>
      <givenName>Virginie</givenName>
      <familyName>Hamel</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-5092-2343</nameIdentifier>
      <affiliation>Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Guichard, Paul</creatorName>
      <givenName>Paul</givenName>
      <familyName>Guichard</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-0363-1049</nameIdentifier>
      <affiliation>Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Manley, Suliana</creatorName>
      <givenName>Suliana</givenName>
      <familyName>Manley</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-4755-4778</nameIdentifier>
      <affiliation>Laboratory for Experimental Biophysics, Institute of Physics, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, SwitzerlandSwiss National Centre for Competence in Research (NCCR) in Chemical Biology, University of Geneva, Geneva, Switzerland</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2020</publicationYear>
  <subjects>
    <subject>ultrastructure expansion microscopy; iSIM;</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2020-01-20</date>
  </dates>
  <resourceType resourceTypeGeneral="Dataset"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/3613906</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3613905</relatedIdentifier>
  </relatedIdentifiers>
  <version>1.0</version>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Datasets containing the segmented expanded centriole particles. The prefix Hs is used to denote particles acquired in synchronized RPE-1 human cells. Otherwise particles were collected from expanded isolated centrioles from &lt;em&gt;Chlamydomoanas reinhardtii&lt;/em&gt;. Resized datasets have uniform voxel size of 14x14x14 nm3 after expansion (56x56x56 nm3 before expansion). Non-resized datasets have 14x14x30 pixel size (56x56x120 nm3 before expansion). All files should be mirrored horizontally/vertically to account for the chirality inversion due to the imaging process&lt;/p&gt;

&lt;p&gt;The channels in different datasets are:&lt;/p&gt;

&lt;ul&gt;
	&lt;li&gt;Chlamy acetylated sample:
	&lt;ul&gt;
		&lt;li&gt;C1: acetylated tubulin-Alexa488&lt;/li&gt;
		&lt;li&gt;C2: aTubulin-Alexa568&lt;/li&gt;
	&lt;/ul&gt;
	&lt;/li&gt;
	&lt;li&gt;Chlamy MonoE sample
	&lt;ul&gt;
		&lt;li&gt;C1: aTubulin-Alexa488&lt;/li&gt;
		&lt;li&gt;C2: GT335-Alexa568&lt;/li&gt;
	&lt;/ul&gt;
	&lt;/li&gt;
	&lt;li&gt;Chlamy PolyE sample
	&lt;ul&gt;
		&lt;li&gt;C1: PolyE-Alexa488&lt;/li&gt;
		&lt;li&gt;C2: aTubulin-Alexa568&lt;/li&gt;
	&lt;/ul&gt;
	&lt;/li&gt;
	&lt;li&gt;Hs sample:
	&lt;ul&gt;
		&lt;li&gt;C1: PolyE-Alexa488&lt;/li&gt;
		&lt;li&gt;C2: acetylated tubulin-Alexa586&lt;/li&gt;
	&lt;/ul&gt;
	&lt;/li&gt;
&lt;/ul&gt;</description>
  </descriptions>
</resource>
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