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Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles

Mahecic, Dora; Gambarotto, Davide; Douglass, Kyle M.; Fortun, Denis; Banterle, Niccoló; Le Guennec, Maeva; Ibrahim, Khalid; Gönczy, Pierre; Hamel, Virginie; Guichard, Paul; Manley, Suliana


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    <dct:title>Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles</dct:title>
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    <dct:description>&lt;p&gt;Datasets containing the segmented expanded centriole particles. The prefix Hs is used to denote particles acquired in synchronized RPE-1 human cells. Otherwise particles were collected from expanded isolated centrioles from &lt;em&gt;Chlamydomoanas reinhardtii&lt;/em&gt;. Resized datasets have uniform voxel size of 14x14x14 nm3 after expansion (56x56x56 nm3 before expansion). Non-resized datasets have 14x14x30 pixel size (56x56x120 nm3 before expansion). All files should be mirrored horizontally/vertically to account for the chirality inversion due to the imaging process&lt;/p&gt; &lt;p&gt;The channels in different datasets are:&lt;/p&gt; &lt;ul&gt; &lt;li&gt;Chlamy acetylated sample: &lt;ul&gt; &lt;li&gt;C1: acetylated tubulin-Alexa488&lt;/li&gt; &lt;li&gt;C2: aTubulin-Alexa568&lt;/li&gt; &lt;/ul&gt; &lt;/li&gt; &lt;li&gt;Chlamy MonoE sample &lt;ul&gt; &lt;li&gt;C1: aTubulin-Alexa488&lt;/li&gt; &lt;li&gt;C2: GT335-Alexa568&lt;/li&gt; &lt;/ul&gt; &lt;/li&gt; &lt;li&gt;Chlamy PolyE sample &lt;ul&gt; &lt;li&gt;C1: PolyE-Alexa488&lt;/li&gt; &lt;li&gt;C2: aTubulin-Alexa568&lt;/li&gt; &lt;/ul&gt; &lt;/li&gt; &lt;li&gt;Hs sample: &lt;ul&gt; &lt;li&gt;C1: PolyE-Alexa488&lt;/li&gt; &lt;li&gt;C2: acetylated tubulin-Alexa586&lt;/li&gt; &lt;/ul&gt; &lt;/li&gt; &lt;/ul&gt;</dct:description>
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