Dataset Open Access

Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles

Mahecic, Dora; Gambarotto, Davide; Douglass, Kyle M.; Fortun, Denis; Banterle, Niccoló; Le Guennec, Maeva; Ibrahim, Khalid; Gönczy, Pierre; Hamel, Virginie; Guichard, Paul; Manley, Suliana

Datasets containing the segmented expanded centriole particles. The prefix Hs is used to denote particles acquired in synchronized RPE-1 human cells. Otherwise particles were collected from expanded isolated centrioles from Chlamydomoanas reinhardtii. Resized datasets have uniform voxel size of 14x14x14 nm3 after expansion (56x56x56 nm3 before expansion). Non-resized datasets have 14x14x30 pixel size (56x56x120 nm3 before expansion). All files should be mirrored horizontally/vertically to account for the chirality inversion due to the imaging process

The channels in different datasets are:

  • Chlamy acetylated sample:
    • C1: acetylated tubulin-Alexa488
    • C2: aTubulin-Alexa568
  • Chlamy MonoE sample
    • C1: aTubulin-Alexa488
    • C2: GT335-Alexa568
  • Chlamy PolyE sample
    • C1: PolyE-Alexa488
    • C2: aTubulin-Alexa568
  • Hs sample:
    • C1: PolyE-Alexa488
    • C2: acetylated tubulin-Alexa586

Files (4.4 GB)
Name Size
Acetyl2_SegParticles.zip
md5:05c67aeb00e4f70fdfc058eed944ed6e
381.7 MB Download
Acetyl_SegParticles.zip
md5:d8beb62ff21051a76487d232839065a6
95.1 MB Download
GT335_SegParticles.zip
md5:64cfdc68bd67e983f5582199b51648ae
1.8 GB Download
Hs_Acetyl_PolyE_SegParticles.zip
md5:14077de82ea55a82224713ec40d257b2
31.1 MB Download
PolyE_SegParticles.zip
md5:0b01ddb7c2e3a014e2ddf68229790c1e
2.1 GB Download
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