January 20, 2020 Dataset Open Access

Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles

Mahecic, Dora; Gambarotto, Davide; Douglass, Kyle M.; Fortun, Denis; Banterle, Niccoló; Le Guennec, Maeva; Ibrahim, Khalid; Gönczy, Pierre; Hamel, Virginie; Guichard, Paul; Manley, Suliana

Datasets containing the segmented expanded centriole particles. The prefix Hs is used to denote particles acquired in synchronized RPE-1 human cells. Otherwise particles were collected from expanded isolated centrioles from Chlamydomoanas reinhardtii. Resized datasets have uniform voxel size of 14x14x14 nm3 after expansion (56x56x56 nm3 before expansion). Non-resized datasets have 14x14x30 pixel size (56x56x120 nm3 before expansion). All files should be mirrored horizontally/vertically to account for the chirality inversion due to the imaging process

The channels in different datasets are:

• Chlamy acetylated sample:
  • C1: acetylated tubulin-Alexa488
  • C2: aTubulin-Alexa568
• Chlamy MonoE sample
  • C1: aTubulin-Alexa488
  • C2: GT335-Alexa568
• Chlamy PolyE sample
  • C1: PolyE-Alexa488
  • C2: aTubulin-Alexa568
• Hs sample:
  • C1: PolyE-Alexa488
  • C2: acetylated tubulin-Alexa586