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Effect of metabolosome encapsulation peptides on enzyme activity, co-aggregation, incorporation and bacterial microcompartment formation

Warren, Martin; Juodeikis, Rokas; Lee, Matt; Mantell, Judith


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  <identifier identifierType="DOI">10.5281/zenodo.3611414</identifier>
  <creators>
    <creator>
      <creatorName>Warren, Martin</creatorName>
      <givenName>Martin</givenName>
      <familyName>Warren</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-6028-6456</nameIdentifier>
      <affiliation>University of Kent</affiliation>
    </creator>
    <creator>
      <creatorName>Juodeikis, Rokas</creatorName>
      <givenName>Rokas</givenName>
      <familyName>Juodeikis</familyName>
      <affiliation>University of Kent</affiliation>
    </creator>
    <creator>
      <creatorName>Lee, Matt</creatorName>
      <givenName>Matt</givenName>
      <familyName>Lee</familyName>
      <affiliation>University of Kent</affiliation>
    </creator>
    <creator>
      <creatorName>Mantell, Judith</creatorName>
      <givenName>Judith</givenName>
      <familyName>Mantell</familyName>
      <affiliation>University of Kent</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Effect of metabolosome encapsulation peptides on enzyme activity, co-aggregation, incorporation and bacterial microcompartment formation</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2020</publicationYear>
  <dates>
    <date dateType="Issued">2020-01-17</date>
  </dates>
  <resourceType resourceTypeGeneral="Audiovisual"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/3611414</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3611413</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;strong&gt;Supplementary Video 1&lt;/strong&gt;. This approach revealed the structures formed by fvMT and BMC shell proteins are remarkably varied in size, shape and volume.&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Supplementary Video 2. &lt;/strong&gt;Recombinant BMCs containing L20-fvMT and -&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Supplementary Video 3.&amp;nbsp;&lt;/strong&gt;&amp;nbsp;Allowing us to quantitate the volume (empty: 54900&amp;plusmn;11013 nm&lt;sup&gt;3&lt;/sup&gt;&amp;nbsp;(n=29); L20-fvMT: 336411&amp;plusmn;177722 nm&lt;sup&gt;3&amp;nbsp;&lt;/sup&gt;(n=60)) and the largest diameter (empty: 61.77&amp;plusmn;15.38 nm (n=29); L20-fvMT: 127.51&amp;plusmn;60.97 nm (n=60)) of these structures&lt;/p&gt;</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>Research Councils UK</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/501100000690</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/RCUK/BBSRC/BB%2FM002969%2F1/">BB/M002969/1</awardNumber>
      <awardTitle>Development of supramolecular assemblies for enhancing cellular productivity and the synthesis of fine chemicals and biotherapeutics.</awardTitle>
    </fundingReference>
  </fundingReferences>
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