3555322
doi
10.5281/zenodo.3555322
oai:zenodo.org:3555322
user-labscribbles
user-sgc-opennotebook
user-openlabnotebooks
Hutchinson, Ashley
Structural Genomics Consortium, University of Toronto
Seitova, Alma
Structural Genomics Consortium, University of Toronto
Arrowsmith, Cheryl
Structural Genomics Consortium, University of Toronto
Edwards, Aled
Structural Genomics Consortium, University of Toronto, MaRS South Tower, 101 College Street, Toronto, Ontario M5G 1L7, Canada and Princess Margaret Cancer Centre and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 1L7, Canada
Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 2019/09/16
Harding, Rachel
Structural Genomics Consortium, University of Toronto
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
Nucleic acid
Protein purification
<p><strong>Project: </strong>Biophysical investigation of purified HTT protein samples</p>
<p><strong>Experiment: </strong>Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material</p>
<p><strong>Date completed:­ </strong>2019/09/16</p>
<p><strong>Rationale:</strong> To purify HTT-HAP40 + Sf9 derived nucleic acid material for cryoEM analysis</p>
Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
Zenodo
2019-09-16
info:eu-repo/semantics/other
3555321
user-labscribbles
user-sgc-opennotebook
user-openlabnotebooks
1579893888.220518
225180
md5:1a907f8a1aea591a89f4ac2911b78685
https://zenodo.org/records/3555322/files/Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 20190916.docx
197986
md5:82b0f69f98991db6e2509126db585dd2
https://zenodo.org/records/3555322/files/Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 20190916.pdf
public
10.5281/zenodo.3555321
isVersionOf
doi