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Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 2019/09/16

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled


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{
  "description": "<p><strong>Project:&nbsp;</strong>Biophysical investigation of purified HTT protein samples</p>\n\n<p><strong>Experiment: </strong>Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material</p>\n\n<p><strong>Date completed:&shy;&nbsp;</strong>2019/09/16</p>\n\n<p><strong>Rationale:</strong>&nbsp;To purify HTT-HAP40 + Sf9 derived nucleic acid material for cryoEM analysis</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@id": "https://orcid.org/0000-0002-1134-391X", 
      "@type": "Person", 
      "name": "Harding, Rachel"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Hutchinson, Ashley"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Seitova, Alma"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Arrowsmith, Cheryl"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto, MaRS South Tower, 101 College Street, Toronto, Ontario M5G 1L7, Canada and Princess Margaret Cancer Centre and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 1L7, Canada", 
      "@type": "Person", 
      "name": "Edwards, Aled"
    }
  ], 
  "url": "https://zenodo.org/record/3555322", 
  "datePublished": "2019-09-16", 
  "keywords": [
    "huntingtin", 
    "Huntington's disease", 
    "Nucleic acid", 
    "Protein purification"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/e20ff128-df1d-40ba-b2bb-c04ad51b17e4/Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 20190916.docx", 
      "encodingFormat": "docx", 
      "@type": "DataDownload"
    }, 
    {
      "contentUrl": "https://zenodo.org/api/files/e20ff128-df1d-40ba-b2bb-c04ad51b17e4/Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 20190916.pdf", 
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  ], 
  "identifier": "https://doi.org/10.5281/zenodo.3555322", 
  "@id": "https://doi.org/10.5281/zenodo.3555322", 
  "@type": "Dataset", 
  "name": "Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 2019/09/16"
}
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