Dataset Open Access

Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 2019/09/16

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled


DCAT Export

<?xml version='1.0' encoding='utf-8'?>
<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:adms="http://www.w3.org/ns/adms#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dct="http://purl.org/dc/terms/" xmlns:dctype="http://purl.org/dc/dcmitype/" xmlns:dcat="http://www.w3.org/ns/dcat#" xmlns:duv="http://www.w3.org/ns/duv#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:frapo="http://purl.org/cerif/frapo/" xmlns:geo="http://www.w3.org/2003/01/geo/wgs84_pos#" xmlns:gsp="http://www.opengis.net/ont/geosparql#" xmlns:locn="http://www.w3.org/ns/locn#" xmlns:org="http://www.w3.org/ns/org#" xmlns:owl="http://www.w3.org/2002/07/owl#" xmlns:prov="http://www.w3.org/ns/prov#" xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" xmlns:schema="http://schema.org/" xmlns:skos="http://www.w3.org/2004/02/skos/core#" xmlns:vcard="http://www.w3.org/2006/vcard/ns#" xmlns:wdrs="http://www.w3.org/2007/05/powder-s#">
  <rdf:Description rdf:about="https://doi.org/10.5281/zenodo.3555322">
    <rdf:type rdf:resource="http://www.w3.org/ns/dcat#Dataset"/>
    <dct:type rdf:resource="http://purl.org/dc/dcmitype/Dataset"/>
    <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://doi.org/10.5281/zenodo.3555322</dct:identifier>
    <foaf:page rdf:resource="https://doi.org/10.5281/zenodo.3555322"/>
    <dct:creator>
      <rdf:Description rdf:about="http://orcid.org/0000-0002-1134-391X">
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">0000-0002-1134-391X</dct:identifier>
        <foaf:name>Harding, Rachel</foaf:name>
        <foaf:givenName>Rachel</foaf:givenName>
        <foaf:familyName>Harding</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Structural Genomics Consortium, University of Toronto</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Hutchinson, Ashley</foaf:name>
        <foaf:givenName>Ashley</foaf:givenName>
        <foaf:familyName>Hutchinson</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Structural Genomics Consortium, University of Toronto</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Seitova, Alma</foaf:name>
        <foaf:givenName>Alma</foaf:givenName>
        <foaf:familyName>Seitova</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Structural Genomics Consortium, University of Toronto</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Arrowsmith, Cheryl</foaf:name>
        <foaf:givenName>Cheryl</foaf:givenName>
        <foaf:familyName>Arrowsmith</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Structural Genomics Consortium, University of Toronto</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Edwards, Aled</foaf:name>
        <foaf:givenName>Aled</foaf:givenName>
        <foaf:familyName>Edwards</foaf:familyName>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Structural Genomics Consortium, University of Toronto, MaRS South Tower, 101 College Street, Toronto, Ontario M5G 1L7, Canada and Princess Margaret Cancer Centre and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 1L7, Canada</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:title>Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 2019/09/16</dct:title>
    <dct:publisher>
      <foaf:Agent>
        <foaf:name>Zenodo</foaf:name>
      </foaf:Agent>
    </dct:publisher>
    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#gYear">2019</dct:issued>
    <dcat:keyword>huntingtin</dcat:keyword>
    <dcat:keyword>Huntington's disease</dcat:keyword>
    <dcat:keyword>Nucleic acid</dcat:keyword>
    <dcat:keyword>Protein purification</dcat:keyword>
    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#date">2019-09-16</dct:issued>
    <owl:sameAs rdf:resource="https://zenodo.org/record/3555322"/>
    <adms:identifier>
      <adms:Identifier>
        <skos:notation rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://zenodo.org/record/3555322</skos:notation>
        <adms:schemeAgency>url</adms:schemeAgency>
      </adms:Identifier>
    </adms:identifier>
    <dct:isVersionOf rdf:resource="https://doi.org/10.5281/zenodo.3555321"/>
    <dct:isPartOf rdf:resource="https://zenodo.org/communities/labscribbles"/>
    <dct:isPartOf rdf:resource="https://zenodo.org/communities/openlabnotebooks"/>
    <dct:isPartOf rdf:resource="https://zenodo.org/communities/sgc-opennotebook"/>
    <dct:description>&lt;p&gt;&lt;strong&gt;Project:&amp;nbsp;&lt;/strong&gt;Biophysical investigation of purified HTT protein samples&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Experiment: &lt;/strong&gt;Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Date completed:&amp;shy;&amp;nbsp;&lt;/strong&gt;2019/09/16&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Rationale:&lt;/strong&gt;&amp;nbsp;To purify HTT-HAP40 + Sf9 derived nucleic acid material for cryoEM analysis&lt;/p&gt;</dct:description>
    <dct:description>Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</dct:description>
    <dct:accessRights rdf:resource="http://publications.europa.eu/resource/authority/access-right/PUBLIC"/>
    <dct:accessRights>
      <dct:RightsStatement rdf:about="info:eu-repo/semantics/openAccess">
        <rdfs:label>Open Access</rdfs:label>
      </dct:RightsStatement>
    </dct:accessRights>
    <dcat:distribution>
      <dcat:Distribution>
        <dct:license rdf:resource="https://creativecommons.org/licenses/by/4.0/legalcode"/>
        <dcat:accessURL rdf:resource="https://doi.org/10.5281/zenodo.3555322"/>
      </dcat:Distribution>
    </dcat:distribution>
    <dcat:distribution>
      <dcat:Distribution>
        <dcat:accessURL>https://doi.org/10.5281/zenodo.3555322</dcat:accessURL>
        <dcat:byteSize>225180</dcat:byteSize>
        <dcat:downloadURL>https://zenodo.org/record/3555322/files/Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 20190916.docx</dcat:downloadURL>
        <dcat:mediaType>application/vnd.openxmlformats-officedocument.wordprocessingml.document</dcat:mediaType>
      </dcat:Distribution>
    </dcat:distribution>
    <dcat:distribution>
      <dcat:Distribution>
        <dcat:accessURL>https://doi.org/10.5281/zenodo.3555322</dcat:accessURL>
        <dcat:byteSize>197986</dcat:byteSize>
        <dcat:downloadURL>https://zenodo.org/record/3555322/files/Large-scale purification of Q23 HTT-HAP40 from Sf9 expression system with contaminating nucleic acid material 20190916.pdf</dcat:downloadURL>
        <dcat:mediaType>application/pdf</dcat:mediaType>
      </dcat:Distribution>
    </dcat:distribution>
  </rdf:Description>
</rdf:RDF>
39
17
views
downloads
All versions This version
Views 3939
Downloads 1717
Data volume 3.4 MB3.4 MB
Unique views 3939
Unique downloads 1515

Share

Cite as