ENERGY DETECTION IN THE FORM OF LIGHT RADIATION AT END OF HUMAN BLOOD COAGULATION CASCADE- THE OPTICAL ABSORPTION OF WATER VS. FIBRIN BURST ENERGY RELEASE

The human blood coagulation cascade had been extensively researched from a biochemistry and molecular perspective. The purpose of this manuscript is to introduce a biophysical phenomenon detected via optical microscopy at the end of the human blood coagulation cascade. This could be described as a sudden energy event in the form of light radiation observed once blood tissue movement stops being attracted to metal iron filings or carbon based graphite particles used as sentinels. Upon close examination of video recordings, the sudden movements of iron particles images coincided with light at the end of the coagulation cascade. A literature search confirmed that both metal filings and graphite particles to possess excellent electrical conductivity. A biophysical light radiation event discharge is hypothesized as result of a burst in the conversion of fibrinogen to fibrin signaling the end of a coagulation cycle; perhaps combined with a piezoelectric effect induced by a sudden clumping of RBCs, or from the optical absorption or water. Method: Metal iron filings or graphite particles were randomly sprinkled on fresh TIBS preparations. The sample was then readily focused and selected particles chosen for video analysis. Equipment used was a video microscope Celestron Model # 44348, glass slides and author’s blood drops. The data was digitally transferred and stored in an Apple computer photo application for further review. Results: When metal iron filings or graphite particles were sprinkled on freshly prepared TIBS slides, video analysis show light radiation emitted at the end of the blood coagulation cascade. Discussion: Since the light radiation emission occurred at the end of the blood coagulation cycle, it is theorized that blood tissue compression could induce piezoelectricity coinciding with energy released by a fibrin burst, or by the optical absorption of water.


Introduction
An optical microscopy method to study in vivo whole fresh human blood drops physical responses to bio-magnetically active and inert material were recently introduced and described (1,2,3). The method demonstrated that when whole blood is smeared onto a glass slide, there is a time window or Wet Time (WT) of approximately 60±20 seconds for a complete water evaporation of the smeared blood tissue. During the WT the human blood components seem to retain their in vivo physical properties, ie: blood cells are documented flowing and maintaining their intrinsic laminar flows. During the WT, foreign bio-magnetically inert material ie: salt ( Fig. 1) or paramagnetic active ie: pepper (Fig. 1A) placed on the slide will either scatter blood components or concentrically repulse the blood tissue.

Temporary In Vivo Blood Smears
As soon as a blood smear is done, water evaporation is observed to start concentrically at the edge of the slide and ending approximately at the center. Molecularly, as the WT comes to an end, the ultimate effect of the blood coagulation cascade will eventually reach a point where the action of the protease enzyme (thrombin) on fibrinogen causes a fibrin burst. The polymerized fibrin together with platelets forms a hemostatic plug or clot (4,5,6,7,8).

Description of Experiments Performed The Temporary In Vivo Blood Smear or (TIBS) Methodology
A finger stick allowed for the milking of two drops of blood, then placed on a clean 25x75x1mm glass slide. The mechanical smear was done as per published instructions from the USA center for disease control (9). There is a time window (TW) of approximately 60±20 seconds for a complete Http://www.granthaalayah.com ©International Journal of Research -GRANTHAALAYAH [203] water evaporation of the smeared blood tissue. The placement of iron metal filings or carbon base graphite particles (approximately midway the TW) were photographed and video recorded by using a Celestron Video Microscope Model #44348 and a MacBook Pro Apple Computer Photo Application for digital images storage for further evaluation.

Time Constraints
Since time is of the essence (Less that 80 seconds) to successfully place the foreign particles on the evaporating smear, the following steps are suggested: • Preset the video microscope into the video mode.
• Have the exogenous particles easily accessible for placement.
• Puncture a finger digit and milk blood.
• Place two blood drops on a slide and prepare a fresh smear.
• Sprinkle the exogenous particles in the center of the evaporating smear.
• Readily mount the smear on the microscope-viewing platform and locate suitable particle(s). • Change the lens magnification for optimal viewing details.
• Begin video recording, with the goal of documenting the evaporation process.
• Select images for analysis and possible presentation.
• Repeat above if unsuccessful.

The placing of Metal Iron Filings and Graphite on TIBS
A total of 15+ experiments were done where metal iron filings were randomly placed on TIBSs. And a total of 2 with graphite particles.

Iron Metal Filings in TIBS Data Presented
Results from experiments are qualitative. In other words, there is zero quantification to report, images are shown as follows:

Metal Iron Filing in TIBS
The figures below represent typical pre and post experiments of the interaction of in vivo human whole blood particles (Red Blood Cells, White Blood Cells and Platelets) with metal iron filings (Figs 3,4,5,6).   S-3: Videoframe at 00:08" of recording. End of contraction-Light rays after full contraction.

Supplemental Image from Previous Experiments Biomagnetism Triggers Blood Coagulation
Images of a biological material (human hair shafts) unilateral biomagnetism had been documented in triggering fibrin (clot) formation. In the same image the contralateral side void of biomagnetism did not trigger clot formation, please see reproduced image from previous research below. compressed RBCs. Demonstrating the after effects of the end of coagulation cycle, where blood tissue was compressed due to the paramagnetic iron metal particles.
Similar example of RBC clumping or accumulation due to paramagnetic attraction. Image denotes post iron particle removal.

Definition of Terms
To aid the reader, the following definitions are included: Biological Material: Are present inside living organisms. They do not contain any metal, ceramic, or synthetic polymer material and have the ability to repair themselves, Biomaterial: Is any combination of natural and synthetic substances (other than drugs) that can be used in the treatment of functions of the body. TIBS: Acronym for "Temporary In Vivo Blood Smear" Limitations: It could be argued that only qualitative images are presented. The measuring of electrical discharges are suggested in future experiments.

Discussion
Due to its medical implications, the human blood coagulation cascade has been extensively researched from a Biochemistry and Molecular Perspective. The temporary preservation of in vivo physical properties of a biological material, such as whole human blood tissue smeared on a glass slide (TIBS) allowed for a demonstration of biological Material cross-talk between endogenous or exogenous magnetically active materials. Demonstrated was RBC deformations and a triggering effect in initiating the blood coagulation cascade. Results from that research were inferred by static images denoting physical changes. In this manuscript, the above findings are now expanded to include video-recordings of in vivo TIBS preparations where whole blood was in direct contact with highly electrically conductive materials such as metal iron filings and graphite particles (10,11). Blood tissue was documented being attracted by bio-magnetic active exogenous materials. The qualitative findings show for the first time light radiation emitted at the end of the coagulation cascade; which happens to coincide with fibrinogen induced fibrin burst. It could be hypothesized that the light emissions could be attributed to a piezoelectric effect from cells clumping due to the magnetic attraction of iron or graphite particles (Figs. 8,9,10,11). This is supported by a previously published experiment demonstration of a piezoelectric effect; where light emission was attributed to the sudden compression of biological material (eukaryotic cells) shifting in laminar flows (12). Also hypothesized is the optical absorption by water phenomenon (13) on the TIBS slide. The methodology herein presented could facilitate and expand, albeit with the proper instrumentation for the evaluation of blood clotting disorders.

Ethics Statement
In compliance. The blood drops tested were from main author.

Conflict of Interests
None to declare.

Funding Sources
None, Research, Ideas, drafting, writing and self-funded by main author.