Rieder, Bernhard
Weihs, Anna
Weidinger, Adelheid
Szwarc, Dorota
Nürnberger, Sylvia
Redl, Heinz
Rünzler, Dominik
Huber-Gries, Carina
Teuschl, Andreas
2018-11-19
<p>Osteoarthritis (OA) is one of the most common causes of disability and represents a major socioeconomic burden. Despite intensive research, the molecular mechanisms responsible for the initiation and progression of OA remain inconclusive. In recent years experimental findings revealed elevated levels of reactive oxygen species (ROS) as a major factor contributing to the onset and progression of OA. Hence, we designed a hydrostatic pressure bioreactor system that is capable of stimulating cartilage cell cultures with elevated ROS levels. Increased ROS levels in the media did not only lead to an inhibition of glycosaminoglycans and collagen II formation but also to a reduction of already formed glycosaminoglycans and collagen II in chondrogenic mesenchymal stem cell pellet cultures. These effects were associated with the elevated activity of matrix metalloproteinases as well as the increased expression of several inflammatory cytokines. ROS activated different signaling pathways including PI3K/Akt and MAPK/ERK which are known to be involved in OA initiation and progression. Utilizing the presented bioreactor system, an OA in vitro model based on the generation of ROS was developed that enables the further investigation of ROS effects on cartilage degradation but can also be used as a versatile tool for anti-oxidative drug testing. </p>
https://doi.org/10.5281/zenodo.3460383
oai:zenodo.org:3460383
eng
Zenodo
https://zenodo.org/communities/fhtw
https://doi.org/10.5281/zenodo.3460382
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Scientific Reports, 8, (2018-11-19)
Osteoarthritis
Bioreactor
Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures
info:eu-repo/semantics/article