Journal article Open Access

Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures

Rieder, Bernhard; Weihs, Anna; Weidinger, Adelheid; Szwarc, Dorota; Nürnberger, Sylvia; Redl, Heinz; Rünzler, Dominik; Huber-Gries, Carina; Teuschl, Andreas


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    "description": "<p>Osteoarthritis (OA) is one of the most common causes of disability and represents a major socioeconomic&nbsp;burden. Despite intensive&nbsp; research, the molecular mechanisms responsible for the initiation&nbsp;and progression of OA remain inconclusive. In recent years&nbsp; experimental findings revealed elevated&nbsp;levels of reactive oxygen species (ROS) as a major factor contributing to the onset and&nbsp; progression&nbsp;of OA. Hence, we designed a hydrostatic pressure bioreactor system that is capable of stimulating&nbsp;cartilage cell cultures&nbsp; with elevated ROS levels. Increased ROS levels in the media did not only lead to&nbsp;an inhibition of glycosaminoglycans and&nbsp; collagen II formation but also to a reduction of already formed&nbsp;glycosaminoglycans and collagen II in chondrogenic mesenchymal&nbsp; stem cell pellet cultures. These&nbsp;effects were associated with the elevated activity of matrix metalloproteinases as well as the&nbsp; increased&nbsp;expression of several inflammatory cytokines. ROS activated different signaling pathways including&nbsp;PI3K/Akt and&nbsp; MAPK/ERK which are known to be involved in OA initiation and progression. Utilizing&nbsp;the presented bioreactor system, an OA in&nbsp; vitro model based on the generation of ROS was developed&nbsp;that enables the further investigation of ROS effects on cartilage&nbsp; degradation but can also be used as a&nbsp;versatile tool for anti-oxidative drug testing.&nbsp;</p>", 
    "language": "eng", 
    "title": "Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures", 
    "license": {
      "id": "CC-BY-4.0"
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    "journal": {
      "issue": "8", 
      "title": "Scientific Reports"
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    "keywords": [
      "Osteoarthritis", 
      "Bioreactor"
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    "publication_date": "2018-11-19", 
    "creators": [
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        "affiliation": "University of Applied Sciences Technikum Vienna", 
        "name": "Rieder, Bernhard"
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      {
        "affiliation": "University of Applied Sciences Technikum Vienna", 
        "name": "Weihs, Anna"
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      {
        "affiliation": "Ludwig Boltzmann Institute for Experimental and Clinical Traumatology", 
        "name": "Weidinger, Adelheid"
      }, 
      {
        "affiliation": "University of Applied Sciences Technikum Vienna", 
        "name": "Szwarc, Dorota"
      }, 
      {
        "affiliation": "Ludwig Boltzmann Institute for Experimental and Clinical Traumatology", 
        "name": "N\u00fcrnberger, Sylvia"
      }, 
      {
        "affiliation": "Ludwig Boltzmann Institute for Experimental and Clinical Traumatology", 
        "name": "Redl, Heinz"
      }, 
      {
        "affiliation": "University of Applied Sciences Technikum Vienna", 
        "name": "R\u00fcnzler, Dominik"
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      {
        "affiliation": "University of Applied Sciences Technikum Vienna", 
        "name": "Huber-Gries, Carina"
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        "affiliation": "University of Applied Sciences Technikum Vienna", 
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