Journal article Open Access

Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures

Rieder, Bernhard; Weihs, Anna; Weidinger, Adelheid; Szwarc, Dorota; Nürnberger, Sylvia; Redl, Heinz; Rünzler, Dominik; Huber-Gries, Carina; Teuschl, Andreas


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  <identifier identifierType="DOI">10.5281/zenodo.3460383</identifier>
  <creators>
    <creator>
      <creatorName>Rieder, Bernhard</creatorName>
      <givenName>Bernhard</givenName>
      <familyName>Rieder</familyName>
      <affiliation>University of Applied Sciences Technikum Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Weihs, Anna</creatorName>
      <givenName>Anna</givenName>
      <familyName>Weihs</familyName>
      <affiliation>University of Applied Sciences Technikum Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Weidinger, Adelheid</creatorName>
      <givenName>Adelheid</givenName>
      <familyName>Weidinger</familyName>
      <affiliation>Ludwig Boltzmann Institute for Experimental and Clinical Traumatology</affiliation>
    </creator>
    <creator>
      <creatorName>Szwarc, Dorota</creatorName>
      <givenName>Dorota</givenName>
      <familyName>Szwarc</familyName>
      <affiliation>University of Applied Sciences Technikum Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Nürnberger, Sylvia</creatorName>
      <givenName>Sylvia</givenName>
      <familyName>Nürnberger</familyName>
      <affiliation>Ludwig Boltzmann Institute for Experimental and Clinical Traumatology</affiliation>
    </creator>
    <creator>
      <creatorName>Redl, Heinz</creatorName>
      <givenName>Heinz</givenName>
      <familyName>Redl</familyName>
      <affiliation>Ludwig Boltzmann Institute for Experimental and Clinical Traumatology</affiliation>
    </creator>
    <creator>
      <creatorName>Rünzler, Dominik</creatorName>
      <givenName>Dominik</givenName>
      <familyName>Rünzler</familyName>
      <affiliation>University of Applied Sciences Technikum Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Huber-Gries, Carina</creatorName>
      <givenName>Carina</givenName>
      <familyName>Huber-Gries</familyName>
      <affiliation>University of Applied Sciences Technikum Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Teuschl, Andreas</creatorName>
      <givenName>Andreas</givenName>
      <familyName>Teuschl</familyName>
      <affiliation>University of Applied Sciences Technikum Vienna</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2018</publicationYear>
  <subjects>
    <subject>Osteoarthritis</subject>
    <subject>Bioreactor</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2018-11-19</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/3460383</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3460382</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/fhtw</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Osteoarthritis (OA) is one of the most common causes of disability and represents a major socioeconomic&amp;nbsp;burden. Despite intensive&amp;nbsp; research, the molecular mechanisms responsible for the initiation&amp;nbsp;and progression of OA remain inconclusive. In recent years&amp;nbsp; experimental findings revealed elevated&amp;nbsp;levels of reactive oxygen species (ROS) as a major factor contributing to the onset and&amp;nbsp; progression&amp;nbsp;of OA. Hence, we designed a hydrostatic pressure bioreactor system that is capable of stimulating&amp;nbsp;cartilage cell cultures&amp;nbsp; with elevated ROS levels. Increased ROS levels in the media did not only lead to&amp;nbsp;an inhibition of glycosaminoglycans and&amp;nbsp; collagen II formation but also to a reduction of already formed&amp;nbsp;glycosaminoglycans and collagen II in chondrogenic mesenchymal&amp;nbsp; stem cell pellet cultures. These&amp;nbsp;effects were associated with the elevated activity of matrix metalloproteinases as well as the&amp;nbsp; increased&amp;nbsp;expression of several inflammatory cytokines. ROS activated different signaling pathways including&amp;nbsp;PI3K/Akt and&amp;nbsp; MAPK/ERK which are known to be involved in OA initiation and progression. Utilizing&amp;nbsp;the presented bioreactor system, an OA in&amp;nbsp; vitro model based on the generation of ROS was developed&amp;nbsp;that enables the further investigation of ROS effects on cartilage&amp;nbsp; degradation but can also be used as a&amp;nbsp;versatile tool for anti-oxidative drug testing.&amp;nbsp;&lt;/p&gt;</description>
  </descriptions>
</resource>
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