Journal article Open Access

Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures

Rieder, Bernhard; Weihs, Anna; Weidinger, Adelheid; Szwarc, Dorota; Nürnberger, Sylvia; Redl, Heinz; Rünzler, Dominik; Huber-Gries, Carina; Teuschl, Andreas


Citation Style Language JSON Export

{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.3460383", 
  "container_title": "Scientific Reports", 
  "language": "eng", 
  "title": "Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures", 
  "issued": {
    "date-parts": [
      [
        2018, 
        11, 
        19
      ]
    ]
  }, 
  "abstract": "<p>Osteoarthritis (OA) is one of the most common causes of disability and represents a major socioeconomic&nbsp;burden. Despite intensive&nbsp; research, the molecular mechanisms responsible for the initiation&nbsp;and progression of OA remain inconclusive. In recent years&nbsp; experimental findings revealed elevated&nbsp;levels of reactive oxygen species (ROS) as a major factor contributing to the onset and&nbsp; progression&nbsp;of OA. Hence, we designed a hydrostatic pressure bioreactor system that is capable of stimulating&nbsp;cartilage cell cultures&nbsp; with elevated ROS levels. Increased ROS levels in the media did not only lead to&nbsp;an inhibition of glycosaminoglycans and&nbsp; collagen II formation but also to a reduction of already formed&nbsp;glycosaminoglycans and collagen II in chondrogenic mesenchymal&nbsp; stem cell pellet cultures. These&nbsp;effects were associated with the elevated activity of matrix metalloproteinases as well as the&nbsp; increased&nbsp;expression of several inflammatory cytokines. ROS activated different signaling pathways including&nbsp;PI3K/Akt and&nbsp; MAPK/ERK which are known to be involved in OA initiation and progression. Utilizing&nbsp;the presented bioreactor system, an OA in&nbsp; vitro model based on the generation of ROS was developed&nbsp;that enables the further investigation of ROS effects on cartilage&nbsp; degradation but can also be used as a&nbsp;versatile tool for anti-oxidative drug testing.&nbsp;</p>", 
  "author": [
    {
      "family": "Rieder, Bernhard"
    }, 
    {
      "family": "Weihs, Anna"
    }, 
    {
      "family": "Weidinger, Adelheid"
    }, 
    {
      "family": "Szwarc, Dorota"
    }, 
    {
      "family": "N\u00fcrnberger, Sylvia"
    }, 
    {
      "family": "Redl, Heinz"
    }, 
    {
      "family": "R\u00fcnzler, Dominik"
    }, 
    {
      "family": "Huber-Gries, Carina"
    }, 
    {
      "family": "Teuschl, Andreas"
    }
  ], 
  "type": "article-journal", 
  "issue": "8", 
  "id": "3460383"
}
181
37
views
downloads
All versions This version
Views 181181
Downloads 3737
Data volume 163.5 MB163.5 MB
Unique views 135135
Unique downloads 3535

Share

Cite as