July 6, 2017 Journal article Open Access

Kamravamanesh, Donya; Pflügl, Stefan; Nischkauer, Winfried; Limbeck, Andreas; Lackner, Maximilian; Herwig, Christoph

MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="041" ind1=" " ind2=" ">
    <subfield code="a">eng</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Photobioreactor Cultivations</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Cyanobacterium</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Poly-β-hydroxybutyrate</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Synechocystis sp. PCC 6714</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Nitrogen and Phosphorus Limitation</subfield>
  </datafield>
  <controlfield tag="005">20200120171112.0</controlfield>
  <controlfield tag="001">3460307</controlfield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Technical University Vienna</subfield>
    <subfield code="a">Pflügl, Stefan</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Technical University Vienna</subfield>
    <subfield code="a">Nischkauer, Winfried</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Technical University Vienna</subfield>
    <subfield code="a">Limbeck, Andreas</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">University of Applied Sciences Technikum Vienna</subfield>
    <subfield code="a">Lackner, Maximilian</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Technical University Vienna</subfield>
    <subfield code="a">Herwig, Christoph</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">1550010</subfield>
    <subfield code="z">md5:8e5e3e4008bdc18ecaf26c349110f341</subfield>
    <subfield code="u">https://zenodo.org/record/3460307/files/Photosynthetic.pdf</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2017-07-06</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire</subfield>
    <subfield code="p">user-fhtw</subfield>
    <subfield code="o">oai:zenodo.org:3460307</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="p">AMB Express</subfield>
    <subfield code="n">7</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">Technical University Vienna</subfield>
    <subfield code="a">Kamravamanesh, Donya</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-fhtw</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">https://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;Poly-&amp;beta;-hydroxybutyrate (PHB) production from CO2&amp;nbsp;has the potential to reduce the production cost of this biodegradable&amp;nbsp;polyesters,&amp;nbsp; and also to make the material more sustainable compared to utilization of sugar feedstocks. In&amp;nbsp;this study the unicellular&amp;nbsp; cyanobacterium, Synechocystis sp. PCC 6714 has been identified as an unexplored potential&amp;nbsp;organism for production of PHB.&amp;nbsp; Synechocystis sp. PCC 6714 was studied under various cultivation conditions and&amp;nbsp;nutritional limitations. Combined effects of&amp;nbsp; nitrogen and phosphorus deficiency led to highest PHB accumulation&amp;nbsp;under photoautotrophic conditions. Multivariate experimental&amp;nbsp; design and quantitative bioprocess development&amp;nbsp;methodologies were used to identify the key cultivation parameters for PHB&amp;nbsp; accumulation. Biomass growth and PHB&amp;nbsp;accumulation were studied under controlled defined conditions in a lab-scale&amp;nbsp; photobioreactor. Specific growth rates&amp;nbsp;were fourfold higher in photobioreactor experiments when cultivation conditions were&amp;nbsp; controlled. After 14 days of&amp;nbsp;cultivation in nitrogen and phosphorus, limited media intracellular PHB levels reached up to 16.4% from&amp;nbsp; CO2.&amp;nbsp;The&amp;nbsp;highest volumetric production rate of PHB was 59 &amp;plusmn; 6 mg L&amp;minus;1 day&amp;minus;1. Scanning electron microscopy of isolated PHB&amp;nbsp;granules of Synechocystis sp. PCC 6714 cultivated under nitrogen and phosphorus limitations showed an average&amp;nbsp;diameter of&amp;nbsp; 0.7 &amp;mu;m. The results of this study might contribute towards a better understanding of photoautotrophic&amp;nbsp;PHB production from&amp;nbsp; cyanobacteria.&lt;/p&gt;</subfield>
  </datafield>
  <datafield tag="773" ind1=" " ind2=" ">
    <subfield code="n">doi</subfield>
    <subfield code="i">isVersionOf</subfield>
    <subfield code="a">10.5281/zenodo.3460306</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.5281/zenodo.3460307</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">publication</subfield>
    <subfield code="b">article</subfield>
  </datafield>
</record>