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Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714

Kamravamanesh, Donya; Pflügl, Stefan; Nischkauer, Winfried; Limbeck, Andreas; Lackner, Maximilian; Herwig, Christoph


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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p>Poly-&beta;-hydroxybutyrate (PHB) production from CO2&nbsp;has the potential to reduce the production cost of this biodegradable&nbsp;polyesters,&nbsp; and also to make the material more sustainable compared to utilization of sugar feedstocks. In&nbsp;this study the unicellular&nbsp; cyanobacterium, Synechocystis sp. PCC 6714 has been identified as an unexplored potential&nbsp;organism for production of PHB.&nbsp; Synechocystis sp. PCC 6714 was studied under various cultivation conditions and&nbsp;nutritional limitations. Combined effects of&nbsp; nitrogen and phosphorus deficiency led to highest PHB accumulation&nbsp;under photoautotrophic conditions. Multivariate experimental&nbsp; design and quantitative bioprocess development&nbsp;methodologies were used to identify the key cultivation parameters for PHB&nbsp; accumulation. Biomass growth and PHB&nbsp;accumulation were studied under controlled defined conditions in a lab-scale&nbsp; photobioreactor. Specific growth rates&nbsp;were fourfold higher in photobioreactor experiments when cultivation conditions were&nbsp; controlled. After 14 days of&nbsp;cultivation in nitrogen and phosphorus, limited media intracellular PHB levels reached up to 16.4% from&nbsp; CO2.&nbsp;The&nbsp;highest volumetric production rate of PHB was 59 &plusmn; 6 mg L&minus;1 day&minus;1. Scanning electron microscopy of isolated PHB&nbsp;granules of Synechocystis sp. PCC 6714 cultivated under nitrogen and phosphorus limitations showed an average&nbsp;diameter of&nbsp; 0.7 &mu;m. The results of this study might contribute towards a better understanding of photoautotrophic&nbsp;PHB production from&nbsp; cyanobacteria.</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Technical University Vienna", 
      "@type": "Person", 
      "name": "Kamravamanesh, Donya"
    }, 
    {
      "affiliation": "Technical University Vienna", 
      "@type": "Person", 
      "name": "Pfl\u00fcgl, Stefan"
    }, 
    {
      "affiliation": "Technical University Vienna", 
      "@type": "Person", 
      "name": "Nischkauer, Winfried"
    }, 
    {
      "affiliation": "Technical University Vienna", 
      "@type": "Person", 
      "name": "Limbeck, Andreas"
    }, 
    {
      "affiliation": "University of Applied Sciences Technikum Vienna", 
      "@type": "Person", 
      "name": "Lackner, Maximilian"
    }, 
    {
      "affiliation": "Technical University Vienna", 
      "@type": "Person", 
      "name": "Herwig, Christoph"
    }
  ], 
  "headline": "Photosynthetic poly-\u03b2-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2017-07-06", 
  "url": "https://zenodo.org/record/3460307", 
  "keywords": [
    "Photobioreactor Cultivations", 
    "Cyanobacterium", 
    "Poly-\u03b2-hydroxybutyrate", 
    "Synechocystis sp. PCC 6714", 
    "Nitrogen and Phosphorus Limitation"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.3460307", 
  "@id": "https://doi.org/10.5281/zenodo.3460307", 
  "@type": "ScholarlyArticle", 
  "name": "Photosynthetic poly-\u03b2-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714"
}
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